长征1-号多器官保存液效果的动物实验研究  

The efficacy of CZ 1 solution in flushing the donor renal transplant for prolonged cold storage

作　　者：郑军华[1] 闵志廉[1] 李玉莉[2] 朱有华[1] 齐隽[1] 叶廷军[2] 李建秋[2] 潘铁文[2] 王孟龙 丁国善[2] 陈卫平[2] 

机构地区：[1]上海第二军医大学长征医院全军泌尿外科中心肾脏移植中心,200003 [2]上海第二军医大学长征医院全军泌尿外科中心,200003

出　　处：《中华泌尿外科杂志》1999年第12期712-714,共3页Chinese Journal of Urology

基　　金：国家自然科学基金;上海市科委重点项目资助

摘　　要：目的　研究长征１ 号（ＣＺ１） 多器官保存液对肾脏低温保存的有效性及移植存活率的影响。　方法　（１） 采用离体肾脏灌注模型，分别检测了低温延时保存后兔肾线粒体呼吸控制率（ＲＣＲ）、Ｎａ＋Ｋ＋ＡＴＰａｓｅ 活性、皮质线粒体Ｃａ２ ＋ 、皮质ＡＴＰ等含量的变化。（２） 采用ＳＤ 大鼠肾脏移植模型，分别观察了低温延时保存４８ 、７２ 小时后再移植，大鼠存活以及移植肾功能恢复情况。　结果　（１）ＣＺ１ 液组保存７２ 小时，其生化指标肾脏线粒体呼吸控制率、皮质线粒体Ｃａ２＋ 、皮质ＡＴＰ含量的变化均优于ＵＷ 液组；（２）经ＵＷ 液和ＣＺ１ 液低温保存供肾４８ 小时，７ 天之内肾功能恢复正常。保存供肾７２ 小时，１４ 天之内肾功能基本接近正常。　结论　长征１ 号多器官保存液对肾脏低温保存效果基本类同于ＵＷ 液，且部分生化学结果优于ＵＷ 液。Objective To evaluate the efficacy of CZ 1 solusion in flushing the donor renal transplant for prolonged cold storage. Methods CZ 1 solusion (Chang Zheng multiorgan preverving solution) has been developed in 1992.Kidneys of New Zeland rabbits were used for the study.How long the kidneys flushed with CZ 1 solusion could be safely cold preserved was investigated.Biochemical changes such as RCR,Na + K + APTase,Ca 2+ concentration of mitochondrial,GSH and ATP were studied. Results The result indicated that CZ 1 solusion is as effective as UW solusion and the kidneys flushed with CZ 1 solution could be safely cold preserved for 72 hours. Conclusions CZ 1 solution is as effective as UW solusion for the cold storage of donor organs and some of the biochemical parameters are even better than those with the use of UW solusion.

关 键 词：移植肾 器官保存 保存液 肾移植 

分 类 号：R699.2[医药卫生—泌尿科学]