芪红胶囊对柯萨奇病毒黏附和穿入的抑制作用  

The inhibited effects of Qihong (QH) capsule on attachment and penetration of coxsackie virus

作　　者：高淑华 宋晓东[2] 孙凯[2] 

机构地区：[1]山西省忻州市人民医院心内科,034000 [2]中国医学科学院阜外心血管病医院教育处

出　　处：《中国药物与临床》2011年第6期627-629,共3页Chinese Remedies & Clinics

基　　金：2008年北京市科技新星(2008B77);高等学校博士学科点专项科研基金(200800231120)

摘　　要：目的观察芪红胶囊(QH)对柯萨奇病毒的抑制作用,并探讨芪红胶囊作用机制。方法首先在HeLa细胞水平研究芪红胶囊对柯萨奇病毒的抑制作用,以利巴韦林作为阳性对照药物。通过细胞活性测定(XTT)实验和空斑形成实验确定芪红胶囊的半数有效剂量(IC50)以及半数毒性剂量(CC50)。在病毒感染后的不同时间段加入芪红胶囊或利巴韦林,观察药物发挥作用的主要时间点。通过黏附实验和穿入实验观察芪红胶囊是否对病毒的吸附和穿入具有抑制作用。结果芪红胶囊具有显著抗病毒作用,XTT实验和空斑形成实验结果显示:芪红胶囊的IC50值分别是:(7.2±0.8)μg/ml和(2.6±0.5)μg/ml;芪红胶囊细胞毒性是利巴韦林的16倍(芪红胶囊的CC50值为:(1648±219)μg/ml,利巴韦林CC50值:(103±14)μg/ml。病毒抑制时程实验提示芪红胶囊主要在病毒感染后0～4h内发挥作用;黏附实验和穿入实验证实芪红胶囊的抗病毒作用主要通过抑制病毒吸附和穿入发挥作用。结论芪红胶囊能够在体外有效抑制病毒对细胞的入侵,且毒性较低,其抗病毒作用主要源于对病毒吸附和穿入的抑制作用。Objective To investigate the inhibited effects of Qihong （QH） capsule and to explore its mechanism.Methods At first,the inhibited effects of QH on coxsackie virus were evaluated in HeLa cell level,with ribavirin as the positive control.The IC50 and CC50 of QH were determined with XTT experiment and plaque forming assay,the main time spots observed with virus-inhibited time-course experiment by adding QH or ribavirin at different time spots after virus infection,the inhibited effects of QH on attachment and penetration of coxsackie virus evaluated with attachment and penetration experiments.Results The results showed the significant anti-virus effect of QH.Outcomes of XXT experiment and plaque forming assay demonstrated that IC50 was （7.2±0.8） μg/ml and （2.6±0.5） μg/ml,respec tively.Furthermore,the cytotoxicity of QH was 16-fold higher than that of ribavirin [CC50 values：QH：（1648±219） μg/ml vs Ribavirin：（103±14） μg/ml].Time-course studies demonstrated that QH mainly exerted its effects at 0-4 h after virus infection.Attachment and penetration experiments proved that QH exerted the anti-virus effects mainly by inhibiting attachment and penetration of coxsackie virus.The results above suggests that QH may be effective in inhibiting the virus invasion to cells in vitro,with relatively low toxicity.Conclusion In addition,QH exerts an anti-virus effect mainly due to its inhibited effect on virus attachment and penetration.

关 键 词：柯萨奇病毒感染 心肌炎 芪红胶囊 

分 类 号：R285.5[医药卫生—中药学]