高效液相色谱法测定化妆品中的染料  

Determination of Dyes in Cosmetic by High Performance Liquid Chromatography

作　　者：王骏[1] 胡俊明[1] 

机构地区：[1]中国预防医学科学院环境卫生监测所,北京100021

出　　处：《色谱》1999年第5期486-487,共2页Chinese Journal of Chromatography

摘　　要：采用反相高效液相色谱法测定化妆品中对苯二胺等 ５ 种染料中间体，以 Ｚｏｒｂａｘ Ｃ８ 为固定相， Ｖ（三乙醇胺）∶ Ｖ（水）∶ Ｖ（乙腈）＝ ０．９５∶９４．０５∶５ 为流动相，加入磷酸调 ｐ Ｈ 值为 ７．７，检测波长为 ２８０ ｎｍ 。样品用体积分数为 ９５％ 的乙醇超声提取，回收率为 ８７％ ～１０７％ （ｎ＝ ６），相对标准偏差为 ２．３％ ～６．４％ 。A reversed\|phase high performance liquid chromatographic method was used to detect 5 dyes including p\|phenylenediamine in cosmetics. A Zorbax C 8 column was used and mobile phase was V(triethanolamine)∶V(water)∶V(acetonitrile)=0.95∶94.05∶5 adjusted to pH 7.7 by phosphoric acid. The wavelength 280 nm was selected. Samples were extracted with 95% ethanol by ultrasonic method. The recoveries were 87%\|107% and the relative standard deviations were 2.3%\|6.4%.

关 键 词：高效液相色谱 化妆品 染料 测定 

分 类 号：TQ658[化学工程—精细化工]