薄层色谱法分析2-丙烯酰胺基-2-甲基丙烷磺酸  

Analysis of 2 Acrylamido 2 Methyl Propane Sulfonic Acid by Thin Layer Chromatography

作　　者：马志[1] 魏天俊[1] 冯光瑛[1] 刘锦贵 赵志杰 

机构地区：[1]河南师范大学化学系,河南新乡453002

出　　处：《色谱》1999年第5期508-509,共2页Chinese Journal of Chromatography

摘　　要：应用薄层色谱法对工业品 ２丙烯酰胺基２甲基丙烷磺酸（简称 Ａ Ｍ Ｐ Ｓ）进行了分析，以硅胶 Ｇ Ｆ２５４作为吸附剂，从几种溶剂系统中优选了 Ｖ（苯）∶ Ｖ （甲醇）＝ ２∶１ 溶液作为展开剂，用 ２５４ ｎｍ 紫外分析仪检测展开后的样品斑点。实验表明： Ａ Ｍ Ｐ Ｓ的 Ｒｆ 为 ０３６，而其主要杂质丙烯酰胺（ Ａ Ｍ）的 Ｒｆ为 ０．６４； Ａ Ｍ Ｐ Ｓ的最小检出限为 ５ μｇ，展开时间约为 ２５ ｍ ｉｎ。方法简便、快速、重现性好，有利于在生产过程中快速分析 Ａ Ｍ Ｐ Ｓ及其所含杂质，从而指导对 Ａ Ｍ Ｐ Ｓ的纯化处理。A method for the analysis of 2 acrylamido 2 methyl propane sulfonic acid(AMPS) by TLC is described. The plate was Silica GF 254 . We selected benzene/methanol as developing agent from several developing systems and fixed the best ratio:V(benzene)∶V(methanol)=2∶1. The experiment indicated that the R f value of AMPS was 0.36 while that of AM(acrylamide), the main impurity in crude AMPS sample, was 0.64 . The minimum detectable limit of AMPS was 5 μg and the developing time was about 25 minutes. Compared with ether/methanol and chloroform/methanol, benzene/methanol system has the advantages of more easily controlling its ratio, better repeatability and much rapid developing rate. This method may be helpful in the control of AMPS in quality.

关 键 词：薄层色谱 丙烯酰胺基 甲基丙烷磺酸 AMPS 测定 

分 类 号：O623.84[理学—有机化学]