花状氧化锌纳米晶的制备及其电化学传感研究  

Synthesis of Flower-Like Zinc Oxide Nanocrystals and Their Electrochemical Biosensing

作　　者：贾向东[1,2] 陆峰[1] 刘燕[1] 朱俊杰[1] 

机构地区：[1]南京大学化学化工学院,生命分析化学教育部重点实验室,南京210093 [2]南京林业大学化学与材料科学系,南京210037

出　　处：《无机化学学报》2011年第6期1150-1154,共5页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.50972058)资助项目

摘　　要：本文以丙三醇为反应溶剂,在氢氧化钾体系中制备了两种花状氧化锌纳米晶,讨论了不同实验条件对氧化锌纳米晶形成影响,通过改变氢氧化钾浓度、反应温度和体系中水含量等条件来调节材料形貌,探讨了花状氧化锌纳米晶可能的形成过程。用X射线粉末衍射、扫描电子显微镜、紫外可见光谱等分析技术对制备的氧化锌纳米晶进行了表征。结果表明两种花状纳米晶均具有六方纤锌矿结构。进一步地,将血红蛋白与花状氧化锌纳米晶混合修饰到电极上构建了新型生物传感器,该传感器能实现血红蛋白的直接电子传递,对过氧化氢具有良好的响应,在1×10^-6-1.2×10^-4 mol.L^-1浓度范围内呈线性关系。Flower-like zinc oxide nanocrystals were synthesized by mixing KOH and ZnCl2 in glycerol solution.The experimental parameters that affected the formation of the nanocrystals were selected,and possible mechanism was discussed.The obtained products were characterized with XRD,SEM techniques and UV-Vis spectroscopy.The results indicated that the flower-like nanocrystals were hexagonal wurtzite structure.In addition,the direct electron transfer between hemoglobin（Hb） and the electrode was realized by immobilizing the protein onto the ZnO nanocrystals.The results showed that the as-fabricated biosensor had a good linear amperometric response to H2O2 in the concentration range from 1×10^-6 to 1.2 ×10^-4 mol·L^-1.

关 键 词：花状氧化锌 纳米材料 直接电化学 生物传感 

分 类 号：O614.241[理学—无机化学] O613.3[理学—化学]