Gd^(3+)对原代培养的小鼠骨髓基质细胞成骨分化和成脂分化的影响(英文)  

Effects of Gd^(3+) on Osteogenic and Adipogenic Differentiation of Mouse Primary Bone Marrow Stromal Cells

作　　者：张金超[1] 谷广其[1] 孙静[2] 张群[1] 郝晓红[1] 王书香[1] 

机构地区：[1]河北大学化学与环境科学学院,河北省化学生物学重点实验室,保定071002 [2]河北大学附属医院B超室,保定071000

出　　处：《无机化学学报》2011年第6期1165-1170,共6页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.20971034);河北省自然科学基金重点项目(No.B2009000161);教育部科学技术研究重点项目(No.208018);河北省留学人员科技活动项目;河北大学自然科学基金资助项目

摘　　要：本文采用MTT法、碱性磷酸酶活性测定、矿化功能的测定以及油红O的染色和定量测定等手段研究了Gd3+对原代培养的小鼠骨髓基质细胞成骨分化和成脂分化的影响。研究结果表明,浓度为1×10-10和1×10-8 mol.L-1的Gd3+对小鼠骨髓基质细胞的增殖没有影响,其他测试浓度下的Gd3+则抑制小鼠骨髓基质细胞的增殖。当Gd3+与小鼠骨髓基质细胞作用7 d时,其对小鼠骨髓基质细胞成骨分化的影响与作用浓度有关,当Gd3+与小鼠骨髓基质细胞作用14 d时,在全部测试浓度范围内,抑制小鼠骨髓基质细胞成骨分化。除1×10-8和1×10-5 mol.L-1外,其他测试浓度下的Gd3+促进小鼠骨髓基质细胞的矿化功能。当Gd3+与小鼠骨髓基质细胞作用10 d时,其抑制小鼠骨髓基质细胞的成脂分化,当Gd3+与小鼠骨髓基质细胞作用16 d时,除1×10-9mol.L-1外,其他浓度的Gd3+也抑制小鼠骨髓基质细胞的成脂分化。实验结果提示,Gd3+可能通过促进骨髓基质细胞的成骨分化、抑制其成脂分化途径起到对骨的保护作用。Gd3+对原代培养的小鼠骨髓基质细胞成骨分化和成脂分化的影响与作用浓度和时间有关,而且,它们是影响Gd3+对骨是损伤还是保护作用转变的关键因素。A series of experimental assays including 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT） test,alkaline phosphatase（ALP） activity measurement,mineralized function,oil red O stain and measurement were employed to assess the effects of Gd^3＋ on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells（BMSCs）.The results indicate that Gd^3＋ has no effect on the proliferation of BMSCs at concentrations of 1×10^-10 and 1×10^-8 mol·L^-1,but inhibits the proliferation at other concentrations.The effect of Gd^3＋ on the osteogenic differentiation depends on concentrations at the 7th day,but Gd^3＋ inhibits the osteogenic differentiation at any concentration at the 14th day.Gd^3＋ can promote the formation of mineralized matrix nodules except at concentrations of 1×10^-8 and 1×10^-5 mol·L^-1.Gd^3＋ can inhibit the adipogenic differentiation at any concentration at the 10th day,but inhibit the adipogenic differentiation except at a concentration of 1×10^-9 mol·L^-1 at the 16th day.These findings suggest Gd^3＋ may have protective effect on bone at appropriate dose by decreasing adipogenic differentiation and promoting osteogenic differentiation of BMSCs.The effects of Gd^3＋ on the osteogenic and adipogenic differentiation of BMSCs depend on the concentration and culture time,moreover,they are pivotal factors for switching the biological effects of Gd^3＋ from damage to protection.

关 键 词：稀土离子 骨髓基质细胞 成骨分化 成脂分化 矿化 

分 类 号：O614.339[理学—无机化学]