一种新型脊柱撑开器的研制及脊髓牵张性损伤动物模型的建立  

ESTABLISHMENT OF TRACTIVE SPINAL CORD INJURY MODEL IN RATS WITH A NOVEL SPINAL DISTRACTOR

作　　者：王文岳[1,2] 杨天府[1] 雷鸣鸣[1,3] 裴福兴[1] 刘雷[1] 

机构地区：[1]四川大学华西医院骨科,成都610041 [2]中国中医科学院广安门医院骨科 [3]成都体育学院运动医学系

出　　处：《中国修复重建外科杂志》2011年第6期705-710,共6页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金面上项目(0040205401265)~~

摘　　要：目的研制一种新型脊柱撑开器并以此建立大鼠脊髓牵张性损伤模型,为脊髓牵张性损伤机制及治疗研究提供可靠的动物模型。方法在既往研究基础上制备一种新型脊柱撑开器。取成年SD大鼠60只,体重250～300 g,随机分为A、B、C、D、E 5组,每组12只。暴露T12～L3脊柱后方结构,咬除T13～L2棘突椎板,显露脊髓。A组仅安放脊柱撑开器,不撑开。B、C、D、E组大鼠,将脊柱撑开器固定于T12～L3椎体横突上,同时行体感诱发电位(somatosensory evoked potential,SEP)监测,其中B、C组脊髓牵张SEP P1下降50%后分别持续5、10 min,D、E组脊髓牵张SEP P1下降70%后分别持续5、10 min,制备T13～L2脊髓牵张性损伤模型。术后1、7 d各组取6只大鼠采用改良Gale联合评分(ICBS)标准进行行为学评分,然后处死大鼠取脊髓行大体观察及HE、尼氏染色,并计数神经元。结果术后1、7 d,A组ICBS评分均为0分,其余各组与A组比较差异均有统计学意义(P<0.05);D、E组ICBS评分显著高于B、C组,差异有统计学意义(P<0.05)。术后1 d B、C、D、E组脊髓表面见不同程度水肿、充血,正常结构形态破坏;7 d时脊髓表面无光泽,与周围组织有不同程度粘连,在牵拉中心段可见硬膜凹陷,均以E组最明显。术后1、7 d B、C、D、E组部分神经元坏死、溶解,尼氏体溶解或消失;7 d时神经元数量随损伤程度加重而逐渐减少,与A组比较差异均有统计学意义(P<0.05),且B、C、D、E组间比较差异均有统计学意义(P<0.05)。结论 新型脊柱撑开器成功制备了不同损伤程度的大鼠脊髓牵张性损伤模型,脊髓牵张SEP P1下降70%持续10 min更符合脊髓牵张性损伤实验研究的需要。Objective To develop a tractive spinal cord injury model in rats with a novel spinal distractor so as to supply the reliable animal model for researching the pathological mechanism and rehabilitation treatment of tractive spinal cord injury.Methods A novel spinal distractor was prepared based on previous study.Sixty adult Sprague Dawley rats（weighing 250-300 g） were randomly divided into 5 groups,12 rats in each group.T12-L3 spinal structures in the rear area were exposed and then T13-L2 spinal cords were revealed via dual laminectomy and kept integrity.In group A,a novel spinal distractor was placed without distraction;in groups B,C,D,and E,the T12-L3 spines were tracted with a novel spinal distractor which put on transverses process of T12-L3 vertebrae.During the tractive period,the somatosensory evoked potential（SEP） was used to monitor spinal cord function.The SEP amplitudes descended 50% and kept distracting for 5 minutes in group B and for 10 minutes in group C,and descended 70% and kept distracting for 5 minutes in group D and for 10 minutes in group E,respectively to establish the tractive spinal cord injury model of T11-L2.The improved combine behavioral score（ICBS） was recorded at 1 and 7 days after injury in 6 rats of each group.The T13-L2 spinal tissue specimens were harvested for the morphological observation by HE and Nissl’s staining and for neurons counting.Results In group A,the ICBS score was 0 at 1 and 7 days after operation,showing significant difference when compared with the scores of the other groups（P 〈 0.05）.The ICBS scores of groups D and E were significantly higher than those of groups B and C（P 〈 0.05）.Edema and hemorrhage were observed in spinal cord surface and normal morphological structures were destroyed at different extent in groups B,C,D,and E at 1 day.There were adherence and congestion between spinal cord surface and peripheral issue without luster at 7 days,and dura depression was observed at the injury section,especially in group E.Necrosis and diss

关 键 词：脊髓牵张性损伤 脊柱撑开器 动物模型 大鼠 

分 类 号：R687.3[医药卫生—骨科学] R-332[医药卫生—外科学]