大田软海绵酸荧光偏振免疫分析方法研究  

Study on Fluorescence Polarization Immunoassay Method for Determination of Okadaic Acid

作　　者：龙再浩[1] 谭曜[1] 陈小青[1] 马中春[1] 

机构地区：[1]宁波出入境检验检疫局,浙江宁波315012

出　　处：《福建分析测试》2011年第3期9-12,共4页Fujian Analysis & Testing

基　　金：浙江省分析测试科技计划项目(2009F70012)

摘　　要：设计使用荧光试剂PDAM与大田软海绵酸单（OA）反应合成了荧光标记物,并在OA单克隆抗体的基础上建立了OA荧光偏振免疫（FPIA）检测方法。该FPIA方法检测限为4.6 ng/mL,检测范围为7.0-88.7 ng/mLI,C50为24.9 ng/mL。本方法操作简单、稳定,有望应用于高通量筛查牡蛎样品中OA含量。A fluorescence polarization immunoassay（FPIA） was developed for detecting okadaic acid（OA） based on monoclonal antibody.The fluorescence-labeled OA were synthesized with PDAM and purified by SPE.The OA FPIA had a dynamic range from 7.0~88.7 ng/mL with IC50 value of 24.9 ng/mL and a detection limit was 4.6 ng/mL.The FPIA method was simple,stable,and showed a potential to apply to determine the level of okadaic acid in mussel.

关 键 词：荧光偏振免疫分析(FPIA) 大田软海绵酸(OA) PDAM 研究 

分 类 号：O657.3[理学—分析化学]