萘普生及其酯类的手性分离和光学纯度测定  

Chiral Separation and Optical Purity Determination of Naproxen and Its Esters by Capillary Electrophoresis with Sulfonated β _ Cyclodextrin as Chiral Selector

作　　者：陆豪杰[1] 阮宗琴[1] 杨永坛[1] 欧庆瑜[1] 

机构地区：[1]中国科学院兰州化学物理研究所,兰州730000

出　　处：《分析测试学报》1999年第6期23-26,共4页Journal of Instrumental Analysis

摘　　要：以磺化β－ 环糊精作为手性分离添加剂， 分别对萘普生、萘普生甲酯和萘普生氯乙酯进行了毛细管电泳手性分离研究， 当以含４０ ｇ／Ｌ 的磺化β－ 环糊精的０ ．０１０ ｍｏｌ／Ｌ 的三羟甲基氨基甲烷－ 柠檬酸为缓冲液（ｐＨ５．０） 时，上述３ 种化合物的手性分离度分别为２ ．１９ 、２ ．６０ 和１ ．９９ 。 手性化合物的出峰时间和校正峰面积的相对标准偏差分别为２ ．８４ ％ 和６ ．２６％ ， 而两对映体的相对出峰时间和相对校正峰面积的相对标准偏差分别为０ ．５２ ％和１ ．８６ ％ 。 对萘普生和萘普生氯乙酯的实际样品进行了光学纯度测定， 测定结果与ＨＰＬＣ 进行了比较。Capillary electrophoretic separation of the weakly acidic naproxen and the neutral enantiomers of its chloroethyl and methyl esters is reported. Sulfonated β _ cyclodextrin was used as the chiral selector for the separation. In case that 0.010 mol·L-1 Tris-citrate buffer(pH 5.0) containing 40 g·L-1 sulfonated β _ cyclodextrin was used as the background electrolyte, the enantioresolutions of the above mentioned three analytes were 2.19, 1.99 and 2.60, respectively. The relative standard deviations(RSD) of migration time and corrected peak area were 2.84% and 6.25%, respectively. With both enantiomers as internal standards for each other, the RSDs of relative migration time and relative peak area of S enantiomer to the R enantiomer were 0.52% and 1.86%, respectively. The optical _ purity results of real naproxen and its chloroethyl ester samples were consistent with those determined by HPLC.

关 键 词：萘普生 萘普生酯类 CE 光学纯度 拆分 测定 

分 类 号：TQ463.4[化学工程—制药化工]