速尿与硫酸卡那霉素联合用药对大鼠内耳毒性的实验观察  

Toxicity of co-administration of furosemide and kanamycin to inner ear in rats

作　　者：袁分钱[1,2] 杨仕明[1] 孙建和[1] 郭维维[1] 刘会占[1] 林昶[2] 

机构地区：[1]解放军总医院耳鼻咽喉头颈外科,解放军耳鼻咽喉研究所耳神经生物中心,北京100853 [2]福建医科大学附属第一医院耳鼻咽喉头颈外科,福州350005

出　　处：《中华耳科学杂志》2011年第1期66-71,共6页Chinese Journal of Otology

基　　金：国家863计划(2007AA02Z150);国家自然科学基金重点项目(30730040),面上项目(30871398,30571017,30000189);海外青年学者合作基金(30628030);北京市自然科学基金(7042061);国家科技支撑计划重大项目(2007BAI18B12.2006BA102B06)

摘　　要：目的探讨速尿与硫酸卡那霉素联合用药对大鼠内耳的毒性作用,为大鼠药物性耳聋动物模型的制备和相关研究提供参考。方法大鼠静脉注射速尿后,立即肌肉注射硫酸卡那霉素,对照组不做处理。用药7天后行听性脑干反应(auditory brainstem response,ABR)阈值检测、耳蜗铺片免疫荧光染色和颞骨常规切片观察,并对耳蜗进行扫描电镜观察。结果联合注射速尿与硫酸卡那霉素后,大鼠ABR阈值明显提高;铺片和切片观察发现内、外毛细胞完全缺失的大鼠,耳蜗支持细胞大部分完整,前庭毛细胞未见损伤;电镜观察发现耳蜗毛细胞纤毛明显受损。结论速尿和硫酸卡那霉素联合用药可导致大鼠听力和耳蜗毛细胞严重受损,但对耳蜗支持细胞和前庭毛细胞损伤较轻,为耳蜗毛细胞损伤后再生等实验研究提供了一种简单、快速而有效的建立大鼠耳聋动物模型的方法。Objective To evaluate the toxicity of co-administration of the loop diuretic furosemide （Fur） and kanamyein （KM） to the inner ear in rats for a reliable model of sensorineural hearing loss. Methods Rats received an intravenous infusion of Fur, followed immediately by an intramuscular injection of KM. Auditory brainstem responses （ABRs） were recorded to monitor the animal＇s hearing at the 7th day after the drug administration.Immunohistoehemical and histopathologieal changes were examined by light microscopy and scanning electron microscopy. Results Immuno-histoehemical and histopathological examination showed Vestibular hair cells and cochlear supporting cells with complete absence of surviving inner and outer cochlear hair cells. Scanning electron microscopy eonfirmed absence of cochlear hair cells. Conclusion Co-administration of furosemide and kanamycin effectively produces profound hearing loss in rats and is an effective method for hearing loss modeling in acute animal experiments.

关 键 词：卡那霉素 速尿 听性脑干反应 毛细胞 

分 类 号：R994.13[医药卫生—毒理学] R764.43[医药卫生—药学]