人脑胶质瘤组织中转化生长因子β1表达与Treg浸润的关系  

Correlation between TGF-β1 expression and Treg cell infiltration in glioma

作　　者：张冠华[1] 蒋小兵[1] 张湘衡[1] 赛克[1] 杨群英[1] 陈忠平[1] 牟永告[1] 

机构地区：[1]华南肿瘤学国家重点实验室中山大学肿瘤防治中心神经肿瘤科,广东广州510060

出　　处：《细胞与分子免疫学杂志》2011年第6期680-682,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30973080);广东省自然科学基金资助项目(8151008901000012)

摘　　要：目的:检测胶质瘤组织中转化生长因子β1(TGF-β1)表达,并分析其与CD4+FOXP3+调节性T细胞(Treg)浸润的关系,探讨Treg浸润的临床意义。方法:采用免疫组织化学双标记的方法检测135例胶质瘤组织(WHOⅠ18,WHOⅡ45,WHOⅢ53,WHOⅣ19)、15例正常脑组织中TGF-β1、Treg(CD4+Foxp3+)的表达。结果:胶质瘤组织中TGF-β1高表达77例,低表达58例,高表达率57.03%,正常脑组织中未见表达。胶质瘤组织中Treg细胞平均密度为2.031个/HP,正常脑组织中未见表达。胶质瘤组织中TGF-β1与Treg细胞表达呈正相关(r=0.294,P<0.01)。高级别胶质瘤组织中TGF-β1,Treg的表达明显高于低级别。胶质瘤组织中Treg细胞表达与患者年龄呈正相关而与性别、KPS评分无关。胶质瘤组织中TGF-β1表达高、低水平组患者总生存率差异有统计学意义(P<0.05),TGF-β1高水平组患者的总生存率低于低水平组(25.97%vs36.21%)。多因素Cox回归模型分析显示TGF-β1表达、Treg细胞数量不是影响胶质瘤患者预后的独立危险因素(P>0.05)。结论:胶质瘤组织中Treg浸润可能与TGF-β1表达有关。高级别胶质瘤组织中TGF-β1,Treg的表达显著高于低级别,高表达组的总生存率显著低于低水平组,但TGF-β1,Treg不能做为影响胶质瘤生存时间的独立预后因素。AIM： To investigate correlation between the expression of TGF-β1 and the amount of Treg cells in glioma,and evaluate their clinical values in predicting the prognosis of glioma.METHODS： Double immunohistochemistry staining was used to detect the expression of TGF-β1,CD4 and Foxp3 in 135 specimens of human gliomas（WHOⅠ18,WHOⅡ45,WHOⅢ53,WHOⅣ19） and 15 normal brain.RESULTS： Of the 135 specimens of glioma,58 showed low TGF-β1 expression and 77（57.03%） showed high TGF-β1 expression while was not expression in normal brain tissue.Average Treg cell density in glioma was 2.031/HP,but there was no expression of Treg in normal brain tissue.Expression of TGF-β1 was positively correlated with the mount of Treg in glioma tissues（r=0.294,P〈0.01）.Compared with the low grade,The levels of TGF-β1 and the amount of Treg cells was significant higher in high-grade glioma,however the mount of Treg had no correlations with Sex,KPS score.The Kaplan-Meier analysis showed that there were significant difference in overall survival（OS） between the TGF-β1 high-expression and low-expression group（P0.001）.Cox multivariate analysis showed that TGF-β1 and Treg were not independent prognostic factors（P〉0.05）.CONCLUSION： Intratumoral of TGF-β1 may relate to the infiltration of Treg cells in glioma tissues.The level of TGF-β1 was obviously increased in high grade.Glioma patients with TGF-β1 or Treg high expression have poorer prognosis,while TGF-β1 and Treg cannot serve as independent prognostic factors of glioma survival time.

关 键 词：胶质瘤 转化生长因子-Β1 调节性T细胞 

分 类 号：R392.11[医药卫生—免疫学]