二极管阵列检测-偏最小二乘吸光光度法同时测定铜锌  

SIMULTANEOUS PHOTOMETRIC DETERMINATION OF ZINC AND COPPER USING DIODE ARRAY DETECTOR AND PARTIAL LEAST SQUARES CALIBRATION MODEL

作　　者：胡晓宇 黎源倩[1] 杨经国[2] 哈元清[2] 

机构地区：[1]华西医科大学卫生检测教研室,成都610041 [2]四川联合大学物理系

出　　处：《理化检验（化学分册）》1999年第10期440-442,444,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金资助课题

摘　　要：研究了以2-(5-溴-2-噻唑氮)5-二乙氨基苯酚(5-Br-PADAP)作显色剂,CCD(Charge Coupled Device,电荷耦合器件)二极管阵列检测分光光度装置采集吸收光谱,用偏最小二乘法解析,同时测定了粮食中铜和锌.方法的线性范围锌为0.05～0.40μg·ml^(-1)·ml^(-1), 铜为0.02～0.60μg·ml^(-1);检出限铜为 0.02μg·ml^(-1),锌为0.05μg·ml^(-1).平均加标回收率锌为93.3%～95.7%,铜为92.2%～192.8%,相对标准差为2.9%～7.0%.Simultaneous photometric determination of zinc and copper using CCD-diode array detector with multivariate calibration of PLS was studied and reported in this paper. The reactions of Cu and Zn with 5-Br-PADAP were studied in detail. The linear ranges of zinc and copper are 0. 05-0.40μg·ml-1 and 0. 02-0. 60μg·ml-1 respectively. The detection limits are 0. 05μg·ml-1 for zinc, and 0. 02μg·ml-1 for copper. The proposed method has been successfully applied to the simultaneous determination of zinc and copper in grain. The average recoveries are found in the range of 93. 3%-95. 7% for zinc and 92. 2%-102. 8% for copper with RSD of 2. 9% -7. 0%. The proposed method is more sensitive, more accurate and faster than the ordinary spectrophotometric method.

关 键 词：测定 吸光光度法 PADAP 铜 锌 

分 类 号：O614.121[理学—无机化学] O614.241[理学—化学]