植物油提取新工艺得到的五味子提取物对小鼠睡眠影响的实验研究  

Study of hypnotic effects of the vegetable oil extract of Fructus schisandrae Chinensis on mice

作　　者：徐仲航[1] 金向群[2] 

机构地区：[1]东北师范大学附属中学,吉林长春130021 [2]吉林大学药学院,吉林长春130021

出　　处：《中国实验诊断学》2011年第5期819-821,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的观察植物油提取新工艺得到的五味子提取物的改善睡眠作用。方法采用植物油提取新工艺得到五味子提取物,昆明种雄性小鼠随机分为对照组及五味子提取物低、中、高剂量组,对照组给予橄榄油20 ml/kg/d灌胃,五味子提取物低、中、高剂量组分别给予五味子提取物150、3006、00 mg/kg/d,连续灌胃15 d,通过直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验和巴比妥钠睡眠潜伏期实验,观察对小鼠直接睡眠、阈剂量戊巴比妥钠睡眠时间、戊巴比妥钠阈下剂量睡眠发生率及巴比妥钠诱导睡眠潜伏期的影响。结果采用植物油提取新工艺得到的五味子提取物可明显延长戊巴比妥钠诱导的小鼠睡眠时间,提高戊巴比妥钠诱导的小鼠睡眠发生率,缩短巴比妥钠诱导的小鼠睡眠潜伏期,对小鼠入睡动物数及睡眠时间均无明显影响。结论采用植物油提取新工艺得到的五味子提取物具有明显改善睡眠作用,有开发利用价值。Objective To investigate the hypnotic effects of vegetable oil extract of Fructus schisandrae Chinensis.Methods vegetable oil extraction extracted of Fructus schisandrae Chinensis.Healthy ICR mice were randomly divided into blank control,high,middle and low dose groups.The effect of sleep improvement was studied by the experiments of sleep-induced directly,prolonging sleeping time induced by pentobarbital sodium,affecting mice sleeping rate under the threshold hypnogenesis dosage of pentobarbital sodium and affecting sleeping delitescence induced by tobarbital sodium.The control group given intragastric administration of olive oil 20 ml/kg/d and the high,middle and low dose were given extracts 600 mg/kg/d for 15 d.Results Oil extraction had no direct effect on the sleep of the mice;could significantly prolong the time of sleep induced by pentobarbital sodium,and increased the number of sleeping mice induced by subthreshold dose of pentobarbital sodium.Conclusion Oil extract of Fructus schisandraeChinensis with the new technique could improve the sleep function of mice.

关 键 词：五味子提取物 改善睡眠作用 戊巴比妥钠 巴比妥钠 小鼠 

分 类 号：R285.5[医药卫生—中药学]