偶发型视网膜色素变性患者CD4^＋CD25^＋调节性T细胞检测  

Detection of CD4^＋CD25^＋ regulatory T cell in patients with primary retinitis, pigmentosa

作　　者：叶辉[1] 夏小平[1] 邓娟[1] 张富程[1] 宋国祥[1] 

机构地区：[1]中山大学附属第三医院眼科,广州510630

出　　处：《中国实用眼科杂志》2011年第6期539-542,共4页Chinese Journal of Practical Ophthalmology

基　　金：基金项目：广东省科技计划项目（20088030301300）

摘　　要：目的检测偶发型视网膜色素变性（RetinitisPigmentosaRP）患者外周血中CD4^＋CD25^＋调节性T细胞的比例，以观察该病病变过程中是否存在自身免疫因素。方法临床实验研究。对2007年9月至2009年8月在中山大学附属第三医院眼科抽取3年内病程偶发型RP患者（19例）、3-5年内病程偶发型RP患者（15例）和健康人（22人）外周静脉血。用流式细胞术检测外周血CD4^＋CD25^＋Treg细胞比例。结果3年内病程偶发型RP患者外周血CD4^＋CD25^＋Treg细胞比例（9．41±d．87）％显著低于健康人外周血CD4^＋CD25^＋Treg细胞比例（13．53±6．28）％，（t=2．795，P〈0．05），差异有统计学意义。3～5年内病程偶发型RP患者外周血CD4^＋CD25^＋Treg细胞比例（11．17±4．90）％与3年内病程偶发型RP患者及健康人外周血CD4^＋CD25^＋Treg细胞比例比较（t值分别为2．795和1．200，P值分别为0．196和2．41，P〉0．05），差异无统计学意义。结论发病早期偶发型RP患者外周血中CD4^＋CD25^＋Treg在淋巴细胞中所占的比例降低，提示在该病早期存在自身免疫现象，为对该病进行免疫学治疗提供了线索。Objeetive To detect ratio of CD4^±CD25^± regulatory T cell in blood of the patients with sporadic type retinitis pigmentosa （RP）. Methods Experimental Stndy Peripheral blood of 19 sporadic type RP patients with 3 years course, 15 sporadic type RP patients with 5 years course, and 22 healthy people were collected. The ratio of CD4^±CD25^± T cell was determined by flow cytometry. Results The ratio of CD4^±CD25^± T cell in sporadic type RP patients with 3 years course （9.41± 4.87）% was far lower than that in healthy controller （13.53± 6.28）%, t =2.795, P 〈0.01. There was no significant difference among the ratio in RP patients with 3-5 years course, in RP patients with less than 3 years course or in healthy controllers. Conclusions The ratio of CD4^±CD25^± T cell in the patients of sporadic type RP decreases in the early stage, mentioning autoimmune factor in the onset of the disease, that provides clue for immunological treatment.

关 键 词：视网膜色素变性 自身免疫 CD4^+CD25^+调节性T细胞 

分 类 号：R392.11[医药卫生—免疫学]