灯盏花素对链脲佐菌素诱导的糖尿病大鼠肾功能的影响  

Effects of Erigeronbreviscapus on Renal Function in Streptozotocin-induced Diabetic Nephropathy in rats

作　　者：孙洁敏[1] 姚美芬[2] 李璐璐[2] 李琦[2] 沈建国[2] 

机构地区：[1]浙江医院内分泌科,杭州310013 [2]浙江大学医学院附属第一医院内分泌科

出　　处：《浙江中西医结合杂志》2011年第5期305-306,310,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:观察灯盏花素对链脲佐菌素(STZ)诱导的糖尿病大鼠肾功能的影响。方法:雄性SD大鼠27只,随机分为正常对照组(C组)、糖尿病模型组(D组)、糖尿病灯盏花素治疗组(DD组)各9只。STZ腹腔注射建立糖尿病模型后,DD组每天给予灯盏花素20mg(/kg.d)灌胃,8周后测定三组大鼠血糖、血尿素氮、血肌酐。摘取左肾称重,右肾浸泡于4%中性福尔马林溶液中,常规HE染色,光镜下观察形态学改变。结果:成模8周时,D组大鼠的体重、血糖与C组比较,差异有统计学意义(P<0.01),DD组与D组大鼠的血糖及体质量比较,差异无统计学意义(P>0.05);D组肾脏肥大指数、血尿素氮、肌酐升高,与C组大鼠比较差异有统计学意义(P<0.01);DD组经灯盏花素治疗8周后,血肌酐下降,但与D组比较差异无统计学意义(P>0.05),尿素氮下降,差异有统计学意义(P<0.01)。肾组织HE染色显示,DD组大鼠肾小球体积较D组有所减小,血管襻开放。结论:灯盏花素不能降低糖尿病大鼠血糖,但能降低大鼠血尿素氮水平,对糖尿病大鼠肾功能有一定保护作用。Objective： To investigate the effects of Erigeronbreviscapus on renal function in rats with Streptozotocin（STZ）-induced diabetic nephropathy（DN）.Methods： A total of 27 male Sprague-Dawley rats were divided into control group,DN group and intervention group,9 in each.Erigeronbreviscapus was given at a dose of 20 mg/kg,i.g per day for 8 weeks in the intervention group and saline was given to the DN group.Serum blood glucose,BUN and CK were measured after treatment;the left kidney was weighed and histology examination was performed on the right kidney.Results： As compared to the control group,the body weight and the level of serum blood glucose was increased in the DN and intervention groups（P0.01）,but no significant difference was noted between the latter 2 groups（P0.05）;the ratio of kidney weight to body weight and serum BUN and CK were increased in the DN group（P0.01）.After treatment,the level of serum CK was deceased without a significant difference（P0.05） and the level of serum BUN was reduced to s significant difference（P0.01） in the intervention group as compared to the DN group.Histological results showed that a decease glomerular volume and a functional structure of glomerular capillary loops in the intervention group.Conclusion： Erigeronbreviscapus can not reduce the level of blood glucose but the level of BUN in diabetic nephropathy rats.

关 键 词：大鼠 糖尿病 肾功能 链脲佐菌素 灯盏花素 

分 类 号：R587.1[医药卫生—内分泌]