呼吸上皮细胞在臭氧气液界面暴露后的氧化损伤效应研究  

Oxidative Damage Effect in Respiratory Epithelial Cells Induced by Exposure to Ozone in Air-Liquid Interface

作　　者：王科霖[1] 管东波[2] 宋宏[1] 

机构地区：[1]中山大学公共卫生学院,广东广州510080 [2]佛山市南海区疾病预防控制中心职业卫生科

出　　处：《环境与健康杂志》2011年第5期397-400,F0003,共5页Journal of Environment and Health

摘　　要：目的探讨臭氧(ozone,O3)对人呼吸上皮细胞(human respiratory epithelial cells,BEAS-2B)的氧化损伤效应及氧化损伤与炎症效应分子分泌的关系。方法利用完全融合并分化完全的人呼吸上皮细胞及petri-PERM透气培养皿建立臭氧体外暴露系统,设立对照(无菌空气)组和高(0.50 mg/m3)、低剂量(0.16 mg/m3)臭氧暴露组以及低剂量(0.16 mg/m3)臭氧暴露+N-乙酰半胱氨酸(NAC,0.01 mol/L)保护组,分别培养1、8 h。测定细胞内GSH、GSSG含量和SOD活力以及细胞培养上清液中IL-2和NO浓度,并观察细胞形态。结果与对照组比较,低剂量和高剂量臭氧暴露组BEAS-2B细胞内GSH/GSSG值较低,细胞内SOD活力以及细胞培养上清中NO和IL-2浓度较高,差异有统计学意义(P<0.05或P<0.01)。与低剂量臭氧暴露组比较,低剂量臭氧暴露+NAC保护组BEAS-2B细胞内GSH/GSSG值较高,细胞内SOD活力以及细胞培养上清中NO和IL-2浓度较低,差异均有统计学意义(P<0.05或P<0.01)。低剂量臭氧暴露+NAC保护组与对照组BEAS-2B细胞内GSH/GSSG值和SOD活力以及细胞培养上清液中IL-2和NO浓度间比较,差异均无统计学意义。在细胞形态上,随着臭氧暴露浓度的升高,BEAS-2B死亡细胞数明显增加,细胞变小变圆;而经NAC保护的细胞生长规则,排列紧密,与对照组基本一致。结论臭氧能对支气管上皮细胞造成氧化损伤,改变细胞内氧化还原状态,并可能诱导支气管上皮细胞合成炎症介质参与气道炎症的启动;而抗氧化剂NAC可以抑制低剂量臭氧暴露对支气管上皮细胞的氧化损伤和炎症介质释放。Objective To investigate the oxidative damage effects of ozone on human respiratory epithelial cells and the relationship between the oxidative damage and the secretion of inflammatory effect molecules. Methods Ozone exposure system in vitro was established with hologamy and completely differentiatted human bronchial epithelial cells and petri-PERM culture dish. Four groups were set：control group（filtrated air）, high dose of ozone group（0.50 mg/m3）, low dose of ozone group （0.16 mg/m3） and low dose of ozone exposure （0.16 mg/m3） ＋ N-acetylcystein （NAC, 0.01 mol/L） protection group, and each group had two time spots （1 and 8 h）. After exposure,the contents of GSH and GSSG in cells,the activities of SOD and the concentrations of NO and IL-2 in the culture media were determined, the change of cell morphology was observed. Results Compared with the control, the ratios of GSH and GSSG in BEAS-2B cells of the low dose and the high dose group were lower, the activities of SOD in cells and the concentrations of NO and IL-2 were higher （P〈0.05 or P〈0.01）. Compared with the low dose of ozone group, the ratio of GSH and GSSG in BEAS-2B cells of low dose of ozone exposure＋ N-acetylcystein protection group was higher, the activity of SOD in cells and the concentration of NO and IL-2 were lower （P〈0.05 or P〈0.01）. There were no significant difference in ratios of GSH and GSSG in BEAS-2B cells, the activities of SOD, and the concentrations of NO and IL-2 in the culture media between the low dose of ozone exposure＋ N-acetylcystein protection group and the control group. Significant morphological differences were found in the cells between the experimental and the control groups. A great number of cells in the experimental group were found to be dead, small and round. While the ceils in the NAC protected group showed little morphological difference from that of the control group. Conclusion Ozone can cause oxidative damage of bronchial epithelial cells, change the intracellular redo

关 键 词：臭氧 气液界面 人呼吸上皮细胞 氧化损伤 

分 类 号：R994.6[医药卫生—毒理学]