拟南芥耐硒突变体的筛选和鉴定  

Screening and identification of selenium-resistant mutants in Arabidopsis

作　　者：慈凌坤[1] 江力[1] 狄东伟[1] 张军[1] 卢云峰[1] 曹树青[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2011年第6期927-930,共4页Journal of Hefei University of Technology：Natural Science

基　　金：安徽省自然科学基金资助项目(070411252X);合肥工业大学大学生创新性实验计划资助项目(2009CXSY208)

摘　　要：文章采用不同质量浓度的亚硒酸钠(Na2SeO3)对拟南芥突变体库中的2 121株拟南芥进行筛选,筛选出29株候选突变体,最终得到2株稳定突变体vse1和vse2,通过表型鉴定和生理生化鉴定确定是突变体。该研究为耐硒基因克隆及功能分析奠定了基础,对于揭示植物耐硒的分子机理具有重要的理论意义。2121 mutant lines of Arabidopsis were screened with the different concentration of sodium selenite（Na2SeO3） for selenium-resistant mutants by root growth analysis.There were 29 mutant lines as candidates and eventually two stable mutant lines vse1 and vse2 were obtained.The phenotype and biochemical identification showed that vse1 and vse2 were selenium-resistant mutants.This study provides a basis for the cloning and functional analysis of selenium-resistant genes,and it is of theoretical significance to reveal the molecular mechanism of the tolerance of plants to selenium.

关 键 词：耐硒 突变体 拟南芥 

分 类 号：Q754[生物学—分子生物学]