叶片中含极芳香物质的蜡梅属植物遗传多样性与亲缘关系  

Genetic diversity and genetic relationships of spacies containing extremely aromatic compounds in leaves of Chimonanthus

作　　者：潘心禾 史小娟[2] 蒋燕锋 程龙军[2] 斯金平[2] 

机构地区：[1]浙江省丽水林业科学研究院中药材研究所,浙江丽水323000 [2]浙江农林大学亚热带森林培育国家重点实验室培育基地天然药物研究开发中心,浙江临安311300

出　　处：《中国中药杂志》2011年第12期1553-1557,共5页China Journal of Chinese Materia Medica

基　　金：浙江省重大科技计划项目(2009C02005)

摘　　要：目的：揭示叶片中含极芳香物质的蜡梅属植物的遗传多样性与亲缘关系,为药材基原鉴别及与开发利用提供基础。方法：采用扩增酶切片段多态性AFLP方法,用POPGENE 32软件进行UPGMA聚类分析。结果：选出10对引物扩增出559条带,多态性条带为226条,多态性条带百分比为36.8%。观测等位基因数、有效等位基因数、Nei′s基因多样性指数和Shannon信息指数在总体水平分别为1.992 6,1.306 5,0.199 2,0.325 1。21个居群间的遗传距离的变异范围在0.039 2~0.289 4。结论：叶片中含极芳香物质的蜡梅属植物遗传多样性低,加强物种与种质资源保护具有重要意义;研究结果从分子水平验证了张若蕙等叶片中含极芳香物质的蜡梅属植物物种分为柳叶蜡梅、浙江蜡梅、山蜡梅、突托蜡梅的正确性。Objective： Species containing extremely aromatic compounds in leaves of Chimonanthus was analyzed to evaluate its genetic diversity and genetic relationships.Method： AFLP molecular marker technique was used in the study,UPGMA cluster analysis was conducted with the software of POPGENE32.Result： Five hundred and fifty-nine bands were amplified by 10 pairs of primers screened,of which 226 bands were polymorphic,and the percentage of polymorphic bands was 36.8%.Observed number of alleles,effective number of alleles,Nei＇s genetic diversity index and Shannon＇s information index were 1.992 6,1.306 5,0.199 2 and 0.325 1,respectively.Genetic distances of the 21 populations were ranged from 0.039 2 to 0.289 4.Conclusion： Species containing extremely aromatic compounds in leaves of Chimonanthus with low genetic diversity play an important role in enhancing the protection of species and germplasm resources.Form the molecular level,the studies demonstrated the correctness of the result by Zhang Ruohui that species containing extremely aromatic compounds in leaves of Chimonanthus were divided into Ch.salicifolius,Ch.Zhejiangensis,Ch.nitens and Ch.grammatus.

关 键 词：蜡梅属 AFLP 遗传多样性 分类 

分 类 号：S685.99[农业科学—观赏园艺]