不同相对分子质量膜处理对黄连解毒汤HPLC特征图谱的影响  

Effect of ultrafiltration by different membranes on HPLC fingerprint of Huanglian Jiedu decorction after being

作　　者：须明玉[1] 周洪亮[2] 郭立玮[1] 

机构地区：[1]南京中医药大学中药复方分离工程重点实验室,江苏南京210029 [2]南京中医药大学药学院,江苏南京210029

出　　处：《中国中药杂志》2011年第12期1582-1586,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30873449);南京中医药大学基础研究项目(08XYC05)

摘　　要：目的:以黄连解毒汤为研究对象,探讨其采用不同相对截留分子质量聚醚砜(PES)超滤膜处理后HPLC特征图谱的变化。方法:用pes-5w,pes-3w,pes-2w,pes-1w,pes-6k的超滤膜处理黄连解毒汤离心液,应用特征图谱技术,分析经各级膜处理后黄连解毒汤成分的种类和含量情况;并测定其浊度、粘度等理化参数。结果:各级膜处理液的药根碱、小檗碱、黄芩素、汉黄芩素逐级变小明显,栀子苷有明显的逐级上升的现象;各药液的理化参数(pH、浊度、电导率)也呈规律性的变化;以离心液为参照,按膜相对分子质量由大到小排序样品的相似度依次为0.993,0.992,0.984,0.982,0.975。结论:本实验运用了膜技术这一较新的分离精制工艺,采用特征图谱全面地反映黄连解毒汤的成分变化,发现各药液成分在种类和含量方面有差异,并探讨了理化参数变化的原因。Objective： To investigate the change of HPLC fingerprint of Huanglian Jiedu decroction.Huanglian Jiedu decorction was ultrafiltrated by different membranes.Method： Different relative molecular weight cutoff membranes were used（pes-5w,pes-3w,pes-2w,pes-1w,pes-6k）to explore its effects on content differences among all samples in HPLC.The turbidity,viscosity and other physical and chemical parameters were measured.Result： Berberine,baicalein and jatrorrhizine became smaller while geniposide increased.Physicall and chemical parameters also showed regular changes.Taking centrifugate as reference,the similarities of the samples treated with high to low molecular weight cutoff membranes were 0.993,0.992,0.984,0.982,0.975,respectively.Conclusion： In this study,the ultrafiltration was used,HPLC fingerprint showed the differences of the samples,and the change of the physical and chemical parameters was explored.

关 键 词：黄连解毒汤 超滤 理化参数 特征图谱 

分 类 号：R284.1[医药卫生—中药学]