鹿蹄草多糖LTC-Ⅱ分离纯化及结构性质研究  

Studies on separation,purification and structure characteristics of a polysaccharide LTC-Ⅱfrom Pyrola corbieri

作　　者：莫正昌[1,2] 吴兰芳[1] 杨娟[1] 王道平[1] 

机构地区：[1]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002 [2]贵州省铜仁职业技术学院,贵州铜仁554300

出　　处：《中国中药杂志》2011年第12期1633-1636,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30760297);国家重点基础研究发展计划项目(2009CB526512)

摘　　要：目的:研究鹿蹄草多糖LTC-Ⅱ的结构性质。方法:热水提取,乙醇沉淀制备鹿蹄草粗多糖,粗多糖经DEAE-纤维素和Sephacryl S-300 HR柱色谱分离纯化。采用高效凝胶渗透色谱法鉴定纯度并测定相对分子质量;紫外、红外、旋光、完全酸水解、高碘酸氧化及Smith降解、部分酸水解、甲基化分析对纯多糖组分进行结构性质研究。结果:得到一多糖纯组分LTC-Ⅱ,其相对分子质量为22 000 Da。单糖组成为阿拉伯糖、甘露糖、葡萄糖、半乳糖,其摩尔比为35.2∶1.0∶13.4∶4.2,LTC-Ⅱ主链由甘露糖、葡萄糖和半乳糖组成,以(1→6)连接的葡萄糖为主,分支侧链由阿拉伯糖、葡萄糖和半乳糖组成,以(1→5)连接的阿拉伯糖为主,葡萄糖和半乳糖位于分子的末端。结论:首次对鹿蹄草多糖LTC-Ⅱ的化学结构进行较深入的研究。Objective： To characterize the structure of polysaccharide LTC-Ⅱ obtained from Pyrola corbieri.Method： The polysaccharide was extracted from P.corbieri by hot water and ethanol precipitation.Crude polysaccharide was purified by DEAE-Cellulose chromatography and Sephacryl S-300 HR column chromatography.The purity and molecular weight of polysaccharide was determined by gel permeation chromatography.UV,IR,optical rotation,complete acid hydrolysis,periodate oxydation,Smith degradation,partial acid hydrolysis and methylation analysis were applied to determine the structural features.Result： A homogeneous fraction LTC-Ⅱ was obtained and its relative molecular mass was 22 000 Da.It consisted of arabinose,mannose,glucose,galactose in the molar ratio of 35.2∶1.0∶13.4∶4.2.LTC-Ⅱ had a backbone consisting glucose,mannose,galactose and mainly contained（1→6）-linkaged glucose.The side chain possessed arabinose,glucose,galactose and mainly contained（1→5）-linkaged arabinose.The terminal sugar were mainly glucose and galactose.Conclusion： Studies on the preliminary characterization of polysaccharide LTC-Ⅱ from P.corbieri for the first time.

关 键 词：鹿蹄草 多糖 分离 纯化 结构分析 

分 类 号：R284[医药卫生—中药学]