白芍中芍药苷及其衍生物的UPLC-MS/MS分析  

Analysis and identification of glycosides in Paeonia lactiflora by UPLC-MS/MS

作　　者：郑敏霞[1] 陈喆[1] 刘培[1] 葛宇清[1] 代群[1] 

机构地区：[1]浙江中医药大学附属第一医院国家中医临床研究基地,浙江杭州310006

出　　处：《中国中药杂志》2011年第12期1641-1643,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30600280)

摘　　要：目的：采用超高效液相色谱与四级杆-线性离子阱串联质谱联用技术（UPLC-Q-Trap MS-MS）对白芍中单萜糖苷类成分进行分析和鉴别。方法：用Acquity UPLC BEH C18色谱柱,以0.1%甲酸水溶液（A）乙腈（B）为流动相梯度洗脱,使用ESI离子源,在负离子模式下采集数据,数据采集范围m/z100~1200,雾化气124.11 kPa,脱溶剂气103.43 kPa,气帘气206.85kPa;离子源温度为450℃;离子喷雾电压为-4.5 kV;结果：白芍中化学成分获得了较好的分离和检测,共推测出6个糖苷类化合物的化学结构;结论：该方法灵敏度高,分离度好,适用于白芍中单萜糖苷类成分的快速分析与结构鉴定。Objective： To investigate the monoterpene glycosides in Paeonia lactiflora by UPLC-MS/MS.Method： An Acquity UPLC BEH C18 column（2.1 mm × 50 mm） with 1.7 μm particle size was used.The mobile phase was composed of acetonitrile and 0.1% formic acid in gradient mode.The flow rate was 0.4 mL·min and the chromatographic run time was 9 min for one run.The mass spectrometer equipped with an eletrospray ion source in negative ion mode.Results： Totally six glycosides were analyzed and identified by the established UPLC-MS/MS method.Conclusion： The method was rapid,sensitive,and extremely useful for rapid identification of glycosides in P.lactiflora.

关 键 词：白芍 单萜糖苷 超高效液相色谱质谱联用法 

分 类 号：R284.1[医药卫生—中药学]