吡啶甲酸铬对热应激异育银鲫血清生化指标的影响  

Effects of chromium picolinate on serum biochemical parameters of heat-stressed carp,Carassius auratus gibelio

作　　者：王晶晶[1] 刘文斌[1] 鲁康乐[1] 蒋阳阳[1] 姜雪姣[1] 梁丹妮[1] 

机构地区：[1]南京农业大学动物科技学院,江苏省水产动物营养重点实验室,南京210095

出　　处：《淡水渔业》2011年第2期51-56,共6页Freshwater Fisheries

基　　金：现代农业产业技术体系建设专项-国家大宗淡水鱼类产业技术体系资金资助(nycytx-49-21);江苏省科技支撑计划(农业)项目-新型渔用复合促生长饲料添加剂关键技术研究与开发资金资助(BE2010394)

摘　　要：选取240尾初始体重为(31.80±1.07)g的异育银鲫(Carassius auratus gibelio),分为4组,分别饲喂在基础饲料中添加0μg/kg(对照组和热应激1组)、300μg/kg(热应激2组)、600μg/kg(热应激3组)吡啶甲酸铬(以Cr3+计)的试验饲料,在室内可控温循环水养殖系统中常温(25℃)下饲养3 d后,进行热应激(31℃)处理,分别测定热应激0 h、12 h、24 h和恢复(25℃)12 h鱼的血清生化指标。结果显示:对照组在25℃下试验36 h内各项血清生化指标变化较小,各时间点差异不显著(P>0.05)。热应激12 h时,各热应激组血清葡萄糖含量均显著升高(P<0.05);24 h时,热应激2组、3组与热应激1组相比呈下降趋势;恢复12 h,热应激3组与对照组相比差异不显著(P>0.05)。热应激12 h时,各热应激组血清胰岛素浓度显著降低(P<0.05);24 h时,热应激2组、3组与热应激1组相比胰岛素含量略有升高,但差异不显著(P>0.05);恢复12 h,热应激2组、3组胰岛素水平继续升高,热应激3组和对照组差异不显著(P>0.05)。各热应激组应激后血清皮质醇浓度和肌酸激酶活性均显著升高(P<0.05),恢复12 h,热应激3组能恢复至与对照组接近(P>0.05)。结果表明,在饲料中添加600μg/kg吡啶甲酸铬,有助于增强鱼体的抗热应激能力。To investigate the effect of chromium picolinate on the regulatory effect of the carp,Carassius auratus gibelio,under normal temperature（25 ℃） and high temperature（31 ℃）,240 fish（mean initial weight of 31.80±1.07 g） were randomly divided into four groups.Fish were fed diets added with 0 μg/kg（as control group and heat-stressed group 1）,300 μg/kg（as heat-stressed group 2）,600 μg/kg（as heat-stressed group 3） chromium picolinate（in terms of Cr3＋）,respectively.After four groups being cultivated for 3 days at 25 ℃,group 1,2 and 3 were heat-stressed for 24 hours at 31 ℃,and then recovered 12 hours after heat stress.Serum biochemical parameters,i.e,glucose,insulin（INS）,cortisol,creatine kinase（CK）,in each group were determined when heat stress lasted for 0 h,12 h,24 h and recovery 12 h.There was no significant change on serum biochemical parameters of control group at 25 ℃（P〈0.05）.Concentration of serum glucose in 3 groups heat-stressed for 12 hours increased significantly（P〉0.05）.Compared with heat-stressed for 24 h group 1,concentration of serum glucose in group 2 and 3 decreased.After recovery 12 hours,the concentration of serum glucose in group 3 was not distinct compared with control group（P〉0.05）.Content of serum INS in 3 groups heat-stressed for 12 hours decreased notablely（P〈0.05）.The concentration of INS in group 2 and 3 was higher than that of group 1 after heat-stress 24 hours（P〉0.05）.The content of INS in group 2 and 3 increased continuously after 12 h recovery,while that in group 3 was not notablely changed compared with the control group（P〉0.05）.The activity of CK and content of cortisol in the serum of 3 heat-stressed groups increased significantly after heat stress（P〈0.05）.Heat-stressed group 3 was close to control group after recovering for 12 hours（P〉0.05）.The results indicated that the addition of 600 μg/kg chromium picolinate could increase the ability of C.auratus gibelio to resist heat stress.

关 键 词：吡啶甲酸铬 热应激 异育银鲫(Carassius auratus gibelio) 血清生化指标 

分 类 号：S963.73[农业科学—水产养殖]