不同时间电针对急性脑出血大鼠氧化应激反应的影响  

Effects of Electroacupuncture at Different Times on Oxidative Stress Reaction in Rats with Acute Cerebral Hemorrhage

作　　者：张英[1] 黄俊涛[2] 段轶轩 汤园园[4] 

机构地区：[1]江汉大学,武汉430056 [2]宜昌市葛洲坝中心医院,湖北443002 [3]武汉市武昌区惠民医院,湖北430060 [4]湖北中医药大学,2009级硕士生武汉430060

出　　处：《上海针灸杂志》2011年第6期418-420,共3页Shanghai Journal of Acupuncture and Moxibustion

摘　　要：目的观察不同时间点针刺治疗对急性脑出血大鼠氧化应激反应的影响。方法 Wistar雄性大鼠随机分为7组,正常组、模型组(模型1组、模型2组、模型3组、模型4组)、3 h电针组和72 h电针组,采用胶原酶Ⅶ诱发大鼠尾壳核脑出血模型。3 h电针组和72 h电针组分别于造模后3 h、72 h时开始针刺,每日1次,共针刺5次。检测血红素氧合酶-1(Heme Oxygenase-1,HO-1)和血红素氧合酶-2(Heme Oxygenase-2,HO-2)。结果造模后脑组织内见大量脑出血,神经纤维缠结、断裂;3 h电针组脑组织仍可见泡沫细胞浸润及出血;72 h电针组脑组织内坏死灶明显缩小、小胶质细胞浸润、极少量出血。正常大鼠脑组织均有HO-1和HO-2表达,脑出血大鼠HO-1表达明显增高,并与时间变化有一定联系。3 h电针组、72 h电针组HO-1均降低,两组之间比较差异无统计学意义(P>0.05)。造模后5 d大鼠脑组织内未出现HO-2表达,造模后8 d出现表达,3 h电针组、72 h电针组均出现HO-2表达,72 h电针组较3 h电针组高,差异有统计学意义(P<0.05)。结论电针可以改善急性脑出血后脑组织出血状况,降低脑出血后氧化应激反应,对急性脑出血后神经元损伤有一定的修复作用。脑出血后72 h电针比3 h电针疗效好,提示急性期脑出血早期应该慎重选择电针治疗。Objective To investigate the effects of electroacupuncture at different time points on oxidative stress reaction in rats with acute cerebral hemorrhage. Methods Wistar rats were randomly allocated to seven groups： normal, model （1, 2, 3 and 4）, 3 h electroacupuncture and 72 h electroacupuncture. A rat model of caudate and putaminal hemorrhage was made using collagenase VII. The 3 h and 72 h electroacupuncture groups were acupunctured at 3 hrs and 72 hrs after model making, respectively. Acupuncture was given once dally, for 5 times. Heme oxygenase-1 （OH-l） and berne oxygenase-2 （OH-2） were measured. Results Cerebral hemorrhage, and neurofibrillary tangles and disruption appeared after model making. Cerebral foam cell infiltration and bleeding was still visible in the 3 h electroacupuncture group. The necrotic foci became small obviously and microglia infiltration and very small amounts of blooding existed in the brain tissue in the 72 h electroacupuncture group. Cerebral OH-1 and OH-2 were both expressed in the normal rats. The OH-1 expression increased significantly and was related to the time change in the cerebral hemorrhage rats. The OH-1 expression decreased in both 3 h and 72 h electroacupuncture groups, but there was no statistically significant difference between the two groups （P〉0.05）. Rat cerebral OH-2 expression did not appear at 5 days after model making but did at 8 days. The OH-2 expression appeared in both 3 h and 72 h electroacupuncture groups and was higher in the 72 h electroacupuncture group than in the 3 h electroacupuncture group. The difference was statistically significant （P〈0.05）. Conclusion Electroacupuncture can reduce brain tissue bleeding and oxidative stress reaction and has a certain repairing effect on injured neurons after acute cerebral hemorrhage. The therapeutic effect of electroacupuncture at 72 hrs after cerebral hemorrhage is better than that at 3 hrs, suggesting that electroacupuncture treatment should be cautiously selected in the early stage of

关 键 词：脑出血 电针 氧化应激 大鼠 

分 类 号：R203[医药卫生—中医学]