CYP2E1基因PstI/RsaI多态性与三氯乙烯药疹样皮炎易感性关系研究  

Research on the relationship between polymorphism of CYP2E1 and dermatitis medicamentosa-like of trichloroethylene

作　　者：越飞[1] 王恰[1] 黄振烈[1] 陈润涛[1] 黄汉林[1] 

机构地区：[1]广东省职业病防治院,广东广州510300

出　　处：《中国职业医学》2011年第3期186-188,共3页China Occupational Medicine

基　　金：国家自然科学基金资助项目(30500401);广东省自然科学基金资助项目(5003556);广东省科技厅计划项目(2004B33701011);广东省医学科研基金项目(A2008067);国家科技支撑计划课题(2006BAI06B02)

摘　　要：目的探讨CYP2E1基因PstI/RsaI多态性与三氯乙烯(TCE)药疹样皮炎易感性的关系,为TCE药疹样皮炎易感人群筛选提供线索和依据。方法采用聚合酶链反应(PCR)扩增后限制性片断长度多态性(RFLP)方法,检测64例TCE药疹样皮炎患者和58名接触对照者中CYP2E1基因PstI/RsaI识别的CYP2E1基因型,比较各种基因型在2组人群中分布情况。结果病例组CYP2E1野生基因型(C1/C1)比例(46.9%)明显高于接触对照组(17.2%),而病例组中其他2种基因型[杂合基因型(C1/C2)和突变纯合基因型(C2/C2)合并]所占比例明显低于对照组(P<0.001)。结论 CYP2E1C1/C1基因型可能是TCE药疹样皮炎的易感基因之一。Objective To study the relationship between polymorphism of CYP2E1 and dermatitis medicamentosa-like of trichloroethylene（DMLT）,and to find some clues for its susceptibility screening.Methods With PCR-RFLP,polymorphism of CYP2E1 gene was determined in 64 patients with DMLT and 58 controls who had contacted with trichloroethylene（TCE） but without DMLT.Then compared the rates of different genotypes in the patient group and the control.The association between genetic polymorphism and susceptibility of DMLT was analyzed.Results The rate of genotype C1/C1 in the patient group was significantly higher than that in the control group,and the Odds Ratio was 4.23.Conclusion The genotype C1/C1 of CYP2E1 may be one of the susceptible factors of DMLT.

关 键 词：三氯乙烯 皮炎 基因多态性 CYP2E1 

分 类 号：R135.1[医药卫生—劳动卫生] R135.7[医药卫生—公共卫生与预防医学]