祛痰止咳颗粒指纹图谱研究  

HPLC Fingerprint of Qutanzhike Granule

作　　者：郑文燕[1] 王晓东[2] 彭维[1] 沈艺[2] 苏薇薇[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]广州市花城制药厂,广东广州510555

出　　处：《中山大学学报（自然科学版）》2011年第3期98-101,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省科技计划资助项目(2010B050100013)

摘　　要：采用HPLC法构建了祛痰止咳颗粒指纹图谱,色谱条件为Elite Hypersil BDS(4.6 mm×250 mm,5μm)色谱柱,以甲醇(A)-0.15%(φ)冰醋酸溶液(B)为流动相,线性梯度洗脱A(15%→75%),流速1.0 mL/min,柱温25℃,检测波长260 nm。该指纹图谱中,确定了19个共有峰,并采用对照品对照法及HPLC-ESI-MS/MS联用技术鉴定出10个黄酮类化合物。构建的指纹图谱,操作简便、准确可靠、重复性较好,为祛痰止咳颗粒质量控制提供了科学依据。HPLC fingerprint of Qutanzhike Granule was established with Elite Hypersil BDS column（4.6 mm×250 mm,5 μm）,methanol（A） and 0.15% acetic acid（B） as mobile phase.The elution profile was： 0~95 min 15% to 75% A in B（linear gradient）.The column temperature was set at 25 ℃ and the flow rate was 1.0 mL/min.The detective wavelength was at 260 nm.19 common peaks were selected as the fingerprint peaks from 12 batches of the production.In the established fingerprint,10 flavonoids were identified by the HPLC-ESI/MS/MS based on MS spetra and standard reference.The method can be used for quality control of Qutanzhike Granule with great precision,accuracy and good reproducibility.

关 键 词：祛痰止咳颗粒 高效液相色谱法 指纹图谱 

分 类 号：R96[医药卫生—药理学]