痰液半定量培养与下呼吸道铜绿假单胞菌感染的关系  

Pseudomonas aeruginosa in lower respiratory tract infection diagnosis with bacterial semi-quantitative culture methods

作　　者：周文[1] 杨肇立[1] 李俊如[1] 李键[1] 杨锦云[1] 任萍[1] 陈旭[1] 黄静[1] 

机构地区：[1]凉山州第一人民医院检验科,四川西昌615000

出　　处：《四川医学》2011年第6期806-808,共3页Sichuan Medical Journal

基　　金：四川省卫生厅科研课题(编号:080160)

摘　　要：目的探讨痰标本细菌半定量培养12级法与下呼吸道铜绿假单胞菌感染诊断的关系。方法涂片观察标本质量、白细胞吞噬菌及白细胞浓集区域优势菌,同时采用细菌半定量12级法培养。查阅分离出铜绿假单胞菌的患者病历,统计分析首次培养的细菌数量与出院诊断的关系。结果白细胞吞噬细菌现象的确存在,但不多见。铜绿假单胞菌128株,其首次培养的相对数量0.1、0.2、≥0.3的确诊率分别为76.9%、78.3%、76.1%;三者的确诊、疑诊、否诊比较,=-52.096,P>0.995。首次培养标本质量为A、B、C级的确诊率分别为91.7%、79.4%、63.4%;A+B级与C级1次的确诊率比较,=4.82,P<0.05;C级1次与C级2次(第1次为C级,第2次未必)的确诊率比较,=0.014,P>0.9。结论按规定要求采集痰标本,若其半定量12级法培养出涂片拟分离菌对应性铜绿假单胞菌的相对数量>该菌口咽部平均携带数量时,应作药敏试验。Objective To study pseudomonas aeruginosa in lower respiratory tract infection diagnosis with bacterial semi-quantitative culture methods（12 degree classification method）.MethodsEvery sputum samples smear to judge the specimen quality,phagocytosis germ of leukocyte,the dominant bacteria of leukocyte concentration region.then culture bacteria with semi-quantitative culture methods.Medical clinical records of Pseudomonas aeruginosa in lower respiratory tract infection case were reviewed,then Statistics analysis of the first culture bacteria colonies amount,specimen quality and discharge diagnosis order.ResultsThere is phagocytosis germ of leukocyte,but that is unusual.There are 128 pseudomonas aeruginosal strain in the first patient sputum samples culture.we can see the relative amount of 12 degree classification method is 0.1,the positive rate is 76.9%,as the relative amount is 0.2,the positive rate is 78.3%,as the relative amount is greater than or equal to 0.3,the positive rate is 76.1%.compared diagnosis rate,suspected cases,false negative results,their Х^2=-52.096,P〈0.995.The specimen quality are A,B and C,the positive rate are respectively 91.7%,79.4%,63.4%.compared diagnosis rate of specimen quality A＋B with C,their Х^2=4.82,P〈0.05.compared diagnosis rate of specimen quality c one times with c two times,their Х^2 are 0.014,P〈0.9.ConclusionThe relative culture mounts of bacterial semi-quantitative culture methods（12 degree classification method） more than that average bacteria carried of oropharynx,we should take drug sensitivity test and report the result to the doctors.

关 键 词：铜绿假单胞 下呼吸道感染 出院诊断 痰半定量培养 

分 类 号：R446.5[医药卫生—诊断学]