恩必普软胶囊对脑卒中后抑郁大鼠海马细胞因子及NF-κB的作用  

Effect of butylphthalide soft capsules on NF-κB and cytokines in hippocampus of rats with post-stroke depression

作　　者：隋汝波[1] 张磊 臧丽娥[1] 

机构地区：[1]辽宁医学院附属第一医院神经内科,锦州121001 [2]急诊科

出　　处：《中国新药杂志》2011年第11期1025-1029,1037,共6页Chinese Journal of New Drugs

基　　金：辽宁省教育厅高等学校科学研究项目(2008406)

摘　　要：目的:探讨恩必普软胶囊(丁苯酞)对卒中后抑郁(post-stroke depression,PSD)大鼠海马组织细胞因子及NF-κB的干预作用。方法:选用健康雄性Sprague-Dawley(SD)大鼠,随机分为单纯缺血组,PSD组,恩必普低(80 mg·kg-1·d-1)、中(160 mg·kg-1·d-1)和高(320 mg·kg-1·d-1)剂量组。进行行为学观察和测试,测定NF-κB的表达及TNF-,αIL-1β和IL-6的含量。结果:与正常对照组比较,其余各组大鼠海马组织NF-κB阳性细胞数及TNF-α和IL-1β的含量出现不同程度增高,而IL-6含量则减少(P<0.01);与单纯缺血组比较,PSD组大鼠海马组织NF-κB阳性细胞数及TNF-α和IL-1β的含量明显增高,而IL-6含量则明显减少(P<0.01);与PSD组比较,恩必普低、中、高剂量组均能不同程度地抑制海马组织NF-κB的表达及减少TNF-α和IL-1β的含量,提高IL-6的含量(P<0.01)。结论:PSD大鼠海马组织细胞因子网络稳态发生改变;恩必普对PSD大鼠海马有保护作用。To determine the effect of butylphthalide soft capsules on NF-KB and cytokines in hippocampus in rats with post-stroke depression （PSD）. Methods： Healthy SD rats were randomly divided into 5 groups. PSD after focal cerebral isehemia was induced, butylphthalide soft capsules at doses of 80, 160 and 320 rag＂ kg-1-d -1 were administered. Behavioral changes of depression were observed, the number of NF-KB-positive ceils was counted, and the contents of TNF-ot, IL,-lβ and IL-6 in hippocampus were measured. Results： Compared with control, NF-KB expression and the contents of TNF-α and IL-lβ significantly increased, and the content of IL-6 significantly decreased （P 〈 0.01 ） in ischemic rats. Compared with focal cerebral isehemia control, NF-KB expression and the contents of TNF-ot and IL-1 [3 significantly increased, and the content of IL-6 significantly decreased （P 〈0. O1 ） in PSD rats. Compared with PSD control, NF-KB expression and the contents of TNF-α and IL-lβ significantly decreased, and the content of IL-6 significantly increased （P 〈 O. 01 ） in rats treated with butylphthalide soft capsules. Conclusion： The steady-state of cytokine network changes in hippoeampus of PSD rats; bulylphlhalide soft capsules have protective effect in the PSD hippocampus.

关 键 词：卒中后抑郁 海马 细胞因子 恩必普软胶囊 丁苯酞 

分 类 号：R972[医药卫生—药品]