奥沙利铂纳米结构脂质载体中主药含量及包封率测定  

Determination of the content and entrapment efficiency of oxaliplatin in drug loaded nano-structured lipid carriers

作　　者：周晖[1] 邱立朋[2] 龙苗苗[2] 李琳[2] 杨磊[2] 闫笑笑[2] 王东凯[2] 

机构地区：[1]中国医科大学附属第一医院,沈阳110016 [2]沈阳药科大学药学院,沈阳110016

出　　处：《中国新药杂志》2011年第11期1030-1033,1019,共5页Chinese Journal of New Drugs

摘　　要：目的:建立奥沙利铂纳米结构脂质载体(oxaliplatin-loaded nanostuctured lipid carriers,OP-NLC)包封率和含量的测定方法。方法:分别采用葡聚糖凝胶色谱法和超滤法分离OP-NLC中游离药物,高效液相色谱法测定包封率及药物含量。结果:超滤法测得药物包封率略高于葡聚糖凝胶色谱法,平均药物回收率和平均加样回收率分别为(99.3±0.6)%和(99.1±2.2)%。OP-NLC平均包封率为(74.2±1.8)%,药物含量为(0.76±0.03)mg·mL-1。结论:超滤法操作简便、准确,可以用于OP-NLC含量和包封率的测定。Objective： To establish an HPLC method for determining entrapment efficiency and content of oxaliplatin in oxaliplatin-loaded nanostuctured lipid carriers （OP-NLC）. Methods： Sephadex G-50 chromatography and ultrafiltration methods were employed to isolate free oxaliplatin, and HPLC was used to determine the entrapmerit efficiency of OP-NLC. Results： Uhrafiltration was more accurate than the other methods. The recovery of ox aliplatin and the recovery of the oxaliplatin sample were （99.3 ± 0.6） % and （99.1 ± 2.2） % , respectively. The average entrapment efficiency of OP-NLC was （74.2 ± 1. 8）% , and the average drug content of OP-NLC was （0.76 ± 0.03 ） mg·mL^- 1. Conclusion ： This method is convenient and accurate for determining the entrapment efficiency and content of OP-NLC.

关 键 词：奥沙利铂 纳米结构脂质载体 包封率 超滤法 

分 类 号：R943.3[医药卫生—药剂学] R979.1[医药卫生—药学]