新型聚乙烯亚胺衍生物在COS-7细胞中转染和毒性的研究  

Transfection and Cytotoxicity Studies of Novel Polyethyleneimine Derivative in COS-7 cells

作　　者：何倩倩[1] 杜子秀[1] 何沐[1] 臧怡[1] 胡振华[1] 王菲[1] 金拓[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《现代生物医学进展》2011年第12期2204-2206,共3页Progress in Modern Biomedicine

基　　金：国家自然科学青年基金项目(200801198)资助

摘　　要：目的:研究以乙二醛为连接剂的聚乙烯亚胺(Polyethyleneimine,PEI)衍生物Polyimine-PEI对非洲绿猴肾癌细胞COS-7的转染活性和细胞毒性的影响。方法:以荧光素酶质粒为报告基因,研究高分子与DNA的复合物在COS-7细胞的转染活性,用MTT方法研究高分子对COS-7细胞的毒性。结果:COS-7细胞实验显示,Polyimine-PEI具有很低细胞毒性,其毒性显著低于PEI25kDa,同时也具有高效输送质粒的能力。结论:Polyimine-PEI是一种新型的高效,低毒在基因治疗领域有相当前景的非病毒载体。Objective：To investigate the transfection efficiency and cell cytotoxicity of Polyethyleneimine（PEI） derivative linking with glyoxal.Methods：Luciferase plasmid was used as the reporter gene to investigate transfection efficiency in COS-7 cells.Cytotoxicity was detected by MTT method.Results：COS-7 cells transfection and cytotoxicity results suggested that the gene carrier（Polyimine-PEI） was less cytotoxicity than that of PEI25kDa,and expressed equal transfection efficiency with PEI25kDa and Lipofectamine 2000.Conclusion：Polyimine-PEI could be a highly efficient and less toxic gene carrier which was promising in gene therapy.

关 键 词：PEI衍生物 COS-7细胞 转染 细胞毒性 

分 类 号：R918[医药卫生—药学]