乳液法静电纺丝PLGA组织工程缓释纤维的研究  

Encapsulation of Proteins in PLGA fibers by Emulsion Electrospinning

作　　者：高小寒[1] 魏蓓蓓[1] 田玲玲[2] 刘一浓[1] 秦明杰[1] 胡振华[1] 吴飞[1] 

机构地区：[1]上海交通大学药学院,上海200240 [2]东华大学纺织学院,上海201620

出　　处：《现代生物医学进展》2011年第12期2224-2226,共3页Progress in Modern Biomedicine

基　　金："国家重大新药创制"针对生物技术药物的相关给药技术平台(2009ZX09310-007)

摘　　要：目的:研究Dextran对蛋白药物的释放影响。方法:将模型蛋白BSA溶解于多糖溶液中,通过W/O乳液法静电纺丝制备缓释纤维。采用MicroBCA法测定该纤维体外释放行为,采用SEC-HPLC检测制备前后蛋白的聚集程度,并与不含多糖的BSA纤维做对照。结果:添加Dextran以后蛋白的包封率由52.68%提高到63.92%,第一天突释不大于药物载量的15%,对蛋白单体的保持达到85%以上。结论:Dextran可以改善一般组织工程纤维中蛋白药物的释放,提高蛋白药物在制剂、贮存、释放过程中的稳定性,增加纤维的载药量。Objective：To investigate the effect of dextran on the releasing behavior of protein drug.Methods：BSA was dissolved in dextran solution,then the fibers was prepared by W/O emulsion electrospinning.The release behavior of the fibers in vitro was detected by MicroBCA method and BSA aggregation was determined by SEC-HPLC.The results were compared with the BSA fiber without dextran.Results：Drug loading for the experiment group reached 63.92 % while control group was only 52.68 %,with first day burst release less than 15 %,BSA monomer preserved more than 85 %.Conclusion：Dextran can improve release behavior of tissue engineering fibers,increase drug loading and drug stability during preparation,storation and release process.

关 键 词：乳液法 静电纺丝 PLGA 组织工程 葡聚糖 

分 类 号：R318[医药卫生—生物医学工程] R918[医药卫生—基础医学]