力竭运动后不同时相大鼠心脏窦房结ADAMTS-1的变化  

The Change in ADAMTS-1 of Sinoatrial Node Cells at Different Time Phases after Exhaustive Exercise in Rats

作　　者：杨红霞[1,2] 常芸[2] 

机构地区：[1]广州体育学院,广州510500 [2]国家体育总局体育科学研究所

出　　处：《中国运动医学杂志》2011年第5期437-441,460,共6页Chinese Journal of Sports Medicine

基　　金：国家体育总局体育科学研究所基本科研业务经费(09-10)

摘　　要：目的:探讨力竭运动后不同时相心脏窦房结带有血小板凝血酶敏感蛋白样模体的解整连蛋白金属蛋白酶-1(ADAMTS-1)mRNA和蛋白表达的变化特点。方法:100只健康成年雄性SD大鼠随机分为10组,每组10只。其中一次力竭游泳运动4组,2周反复力竭游泳运动4组,相应的安静对照组2组。安静对照组不运动。反复力竭各组大鼠尾部负重约3%体重,进行每周6天、每天1次、每次2小时左右、共2周的力竭游泳运动。一次力竭运动各组大鼠在正常喂养2周后进行一次性力竭游泳运动,方案同反复力竭组。分别于力竭运动后0、4、12及24小时取材,应用激光显微切割技术定位并收集窦房结细胞,通过实时荧光定量PCR、免疫荧光组化和图像分析技术测试大鼠心脏窦房结ADAMTS-1 mRNA和蛋白表达的变化。结果:一次力竭和反复力竭运动后4小时、12小时、24小时大鼠心脏窦房结ADAMTS-1 mRNA含量均显著低于其安静对照组(P<0.05);一次力竭和反复力竭运动后4小时、12小时、24小时心脏窦房结ADAMTS-1蛋白含量均显著低于其安静对照组(P<0.05),且力竭运动后即刻ADAMTS-1蛋白表达显著高于对照组(P<0.01)。结论:力竭运动后即刻心脏窦房结ADAMTS-1蛋白水平呈高表达,力竭运动后其它时相窦房结ADAMTS-1在mRNA和蛋白水平呈低表达,可能导致窦房结损伤。Objective This paper discusses the mRNA and protein expression of a disintegrin-like and metalloproteinase with thrombospondin type1 motifs（ADAMTS-1）of sinoatrial node（SAN）at different time phrases after exhaustive exercise.Methods One hundred healthy adult male SD rats were equally assigned into 10 groups,including 4 groups undergoing single exhaustive swimming,4 groups undergoing 2-week repeated exhaustive swimming,and 2 control groups.Rats underwent swimming with loading（3% of body weight）on their tails.2-hour exhaustive swimming was carried out for the repeated swimming groups 6 days per week for a total of 2 weeks.After 2-week normal feeding,2-hour exhaustive swimming was carried out for the single exhaustive swimming group.The samples were drawn from rats immediately,and at 4,12,and 24 hours after exhaustive swimming.The SAN cells were spotted and collected with the approach of laser microdissection（LMD）.The mRNA and protein expression of ADAMTS-1 in SAN were analyzed by real-time fluorescent quantitative PCR,immunochemistry and image analysis.Results The ADAMTS-1 mRNA and protein in SAN of the single exhaustive swimming group and repeated exhaustive swimming group at 4,12,and 24 hours after exhaustive swimming were significantly lower than that of control group（P 0.05 and P 0.05）,especially at 4 hours after;however,expression of ADAMTS-1 protein was significantly higher than that of control group（P 0.01）.Conclusion The ADAMTS-1 protein in SAN is highly expressed right after exhaustive exercise,whereas lower level of its transcription and protein reveals in the following other time-phrases,which probably impairs the sinoatrial node.

关 键 词：力竭运动 窦房结 金属蛋白酶-1 

分 类 号：R87[医药卫生—运动医学]