骨髓间充质干细胞抗辐射基因Survivin和HO-1的表达  

Expression of Radioresistant Genes Survivin and HO-1 in Mesenchymal Stem Cells

作　　者：吴冰[1] 王艳春[1] 韦艳[1] 周雅丽[1] 张玲[1] 张茜[1] 王存邦[1] 白海[1] 

机构地区：[1]兰州军区兰州总医院血液病研究所,甘肃兰州730050

出　　处：《中国实验血液学杂志》2011年第3期805-808,共4页Journal of Experimental Hematology

基　　金：军队临床高新技术重大项目资助(编号2010GXJS014);甘肃省中青年科技研究基金项目资助(编号1007RJYA018)

摘　　要：本研究探讨人骨髓间充质干细胞抗辐射基因survivin和HO-1的表达。采用Fircoll密度梯度离心法自人骨髓中分离MSC并进行纯化和扩增,通过流式细胞术检测其表面标志并进行鉴定;用地塞米松、胰岛素、3-异丁基-1-甲基黄嘌呤(IBMX)、吲哚美辛等定向诱导BM-MSC分化为脂肪细胞;RT-PCR检测MSC中survivin和HO-1基因表达。结果表明:体外分离培养的MSC中CD34、HLA-DR表达为阴性,CD71、CD44表达为阳性,并且可诱导为脂肪细胞。RT-PCR检测MSC显示有survivin和HO-1抗辐射基因表达。结论 :MSC具有较低的辐射敏感性,这可能与抗辐射基因survivin和HO-1表达相关。This study was aimed to investigate the expression of radioresistant genes survivin and HO-1 in mesenchymal stem ceils （MSC）. Human bone marrow MSC were isolated and enriched by Fircoll density gradient centrifugnation, then identified by flow cytometry. MSC were induced with dexamethasone, insulin, 3-isobutyl-1-methyl-xanthine （IBMX） and indomethacin to differentiate into adipocytes. Then the expression of survivin and HO-1 in MSC was dectected by RT-PCR. The results indicated that the expressions of surface antigen CD34 and HLA - DR in MSC in vitro were negative while the expressions of CD44 and CD71 were positive. MSC could be differentiated into adipocytes by inductor. RT-PCR showed the expression of radioresistant genes survivin and HO-1 in MSC. It is concluded that MSC have lower sensitivity to radiation, which may associate with the expression of radioresistant genes survivin and HO-1 in MSC.

关 键 词：间充质干细胞 SURVIVIN 血红素氧合酶-1 辐射敏感性 

分 类 号：R818.03[医药卫生—放射医学]