甲醛对PC12细胞内源性H_2S生成的影响  

Effect of formaldehyde on endogenous hydrogen sulfide production in PC12 cells

作　　者：尹蔚兰[1] 何剑琴[1] 方恒荣[1] 杨丝丝[1] 胡弼[1] 唐小卿[1] 

机构地区：[1]南华大学生理学教研室,湖南衡阳421001

出　　处：《中国药理学通报》2011年第7期957-960,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No81071005);湖南省教育厅资助项目(No06C700)

摘　　要：目的观察甲醛对PC12细胞内源性硫化氢(hydrogensulfide,H2S)生成的影响,以探讨甲醛损伤PC12细胞的新机制。方法 RT-PCR方法检测PC12细胞的胱硫醚-β-合酶(cystathionine-beta-synthase,CBS)mRNA的表达;亚甲基蓝分光光度计法检测PC12细胞CBS活性及PC12细胞内源性H2S的含量;MTT法观察PC12细胞的存活率。结果甲醛可以抑制PC12细胞CBS的表达及其活性,减少内源性H2S的生成;甲醛可以明显地降低PC12细胞的存活率。结论甲醛能抑制CBS的表达和活性,减少内源性H2S生成,这可能与其损伤PC12细胞有关。Aim To observe the effect of formaldehyde on the production of endogenous hydrogen sulfide（H2S） in PC12 cells and to explore the novel molecular mechanisms of formaldehyde-induced damage in PC12 cells.Methods The expression of cystathionine-beta-synthase（CBS） mRNA in PC12 cells was determined by RT-PCR.The production of H2S and the activity of CBS in PC12 cells were detected by methylene blue spectrophotometric method.The survival of PC12 cells was observed by MTT assay.Results Formaldehyde inhibited the expression of CBS mRNA and the activity of CBS,and decreased the production of endogenous H2S in PC12 cells.Formaldehyde significantly reduced the survival rate of PC12 cells.Conclusion Formaldehyde inhibits the expression and activity of CBS,as well as endogenous H2S production in PC12 cells,which may be associated with formaldehyde-induced PC12 cells damage.

关 键 词：甲醛阿尔采末病 硫化氢 胱硫醚-β-合酶 PC12细胞 细胞存活率 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R745.702.2[医药卫生—基础医学]