丹参乙酸镁对人胚肾细胞内血红素加氧酶表达的影响  

Effects of magnesium lithospermate B on the expression of heme oxygenase-1 in HEK293T cells

作　　者：任贤 侯瑞英[2] 屈健[2] 沈杰[2] 周宏灏[2] 刘昭前[2] 

机构地区：[1]上海绿谷制药有限公司,上海201304 [2]中南大学临床药理研究所遗传药理学湖南省重点实验室

出　　处：《中国临床药理学与治疗学》2011年第5期481-487,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(30873089);湖南省自然科学基金重点项目(08JJ3058);上海绿谷制药有限公司委托项目

摘　　要：目的:以人胚肾细胞(HEK293T)为研究对象,进一步证实丹参乙酸镁(magnesium litho-spermate B,LAB)对氧化应激下细胞内活性氧(reactive oxygen species,ROS)产生的影响;以血红素加氧酶-1(heme oxygenase,HO-1)为靶点,观察LAB对高糖诱导下HO-1表达的影响。方法:运用流式细胞仪检测细胞内ROS水平,RT-PCR和Western blotting分别检测高糖和LAB干预下,HEK293T细胞内HO-1基因mR-NA和蛋白表达水平。结果:高糖和H2O2可使HEK293T细胞内ROS产生明显增多,LAB能够明显抑制应激状态时HEK293T细胞内ROS产生。HEK293T细胞给予高糖刺激后,HO-1 mR-NA表达和蛋白质表达均上调,于24 h达到高峰。HEK293T细胞预先30 min给予10、50和100 mol/L 3个不同浓度的LAB干预后,与高糖对照组比较,HO-1 mRNA和蛋白质表达水平均有显著性的增加。结论:LAB不但可以直接清除氧化应激下细胞内过多的ROS,而且还可以通过激活下游基因HO-1的表达而发挥抗氧化作用。AIM： To further confirm the effects of magnesium lithospermate B（LAB） on the expression of heme oxygenase-1（HO-1） in HEK293T cells induced by high glucose. METHODS： The intracellular reactive oxygen species in cells was determined by flow cytometry.The expression levels of HO-1 mRNA and HO-1 protein in HEK293T cells treated with high glucose and LAB were determined by quantitative reverse transcription polymerase chain reaction（QRT-PCR） and western blotting.RESULTS：Flow cytometry showed that the ROS concentration was increased markedly in HEK293T cells induced by high glucose and H2O2 and LAB inhibited the ROS generation of HEK293T cells.The expression levels of HO-1 mRNA and protein were increased significantly in HEK293T cells treated with high glucose and reached the peak value at 24 h.Compared with high glucose group,the expression levels of HO-1 mRNA and protein were obviously enhanced after cells were treated with 10,50,100 mol/L LAB.CONCLUSION： LAB not only can directly clear up the excess ROS in cells under oxidative stress,but also it play a antioxidant role via an increase of downstream gene HO-1 expression.

关 键 词：丹参乙酸镁 活性氧 血红素加氧酶 人胚肾细胞 

分 类 号：R965.2[医药卫生—药理学]