纳洛酮对丙泊酚致小鼠学习记忆障碍、催眠作用的影响  

Effect of Naloxone on Propofol-induce Learning Deficit and Dysmnesia and Hypnosis of Mice

作　　者：江维[1] 谢伟[1] 杨婷[2] 张春雨[1] 崔胜[1] 王娇[1] 张科学[1] 闫肃[1] 戴体俊[1] 

机构地区：[1]徐州医学院麻醉学院,徐州221002 [2]徐州医学院华方学院,徐州221000

出　　处：《航空航天医学杂志》2011年第3期267-268,271,共3页Journal of Aerospace medicine

基　　金：国家自然科学基金(批号:30471657;39970715);江苏省自然科学基金(批号:BK2001143);徐州医学院院课题(批号:09KJ08)

摘　　要：目的:探讨纳洛酮对丙泊酚致小鼠学习记忆障碍以及催眠作用的影响。方法:小鼠腹腔注射不同剂量的丙泊酚(10,100 mg·kg-1)建立模型,跳台、避暗实验中观察不同剂量的纳洛酮sc对小鼠跳台潜伏期(stepdown latency,SDL)、步入潜伏期(step through latency,STL)和错误次数的影响;催醒实验中观察不同剂量的纳洛酮皮下注射(subcutaneously,sc)对小鼠睡眠潜伏期和持续时间(sleeping time,ST)的影响。结果:跳台、避暗实验中,用药后第1 d,纳洛酮0.5 mg·kg-1sc能够延长丙泊酚所致记忆障碍小鼠的SDL、STL,减少错误次数(P<0.05;P<0.01),用药后第2、3、4 d,各组SDL、STL和错误次数的差异均无显著性(P>0.05);催醒实验中,纳洛酮0.5 mg·kg-1sc对丙泊酚催眠小鼠的潜伏期和ST没有明显影响(P>0.05),纳洛酮1.2 mg·kg-1sc能够延长丙泊酚催眠小鼠的潜伏期,缩短其ST(P<0.05;P<0.01)。结论:纳洛酮可以改善丙泊酚所致的学习记忆障碍,可以减轻丙泊酚的催眠作用。Objective：To investigate the effect of naloxone on propofol-induced learning deficit and dysmnesia and hypnosis of mice. Methods：Animal models were established by intraperitoneal injecting（ip） of propofol（10 and 100 mg·kg^-1）.Normal saline or different doses of naloxone were subcutaneously injected（sc）,then the step down latency（SDL）,error times in step down test,step through latency（STL）,error times in step through test were observed and recorded;and sleeping latency and time（ST） in analeptic test were recorded. Results：In step down test and step through test,the first day after treatment,naloxone 0.5 mg·kg^-1（sc） increased the SDL and STL,and decreased the error times of mice treated with propofol（P0.05;P0.01）;2,3,4 days after drug,there was no significant difference in SDL,STL,and error times of each group（P0.05）;In analeptic test,naloxone 0.5 mg·kg^-1（sc） on propofol hypnosis in mice did not affect the latency and ST（P 0.05）,naloxone 1,2 mg·kg^-1（sc） extend the sleeping latency and decreased the ST（P0.05;P0.01）. Conclusions：naloxone could improve propofol-induced learning deficit and dysmnesia and abate the hypnosis of propofol.

关 键 词：丙泊酚 纳洛酮 避暗实验 跳台试验 学习记忆障碍 催眠 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]