Wistar大鼠带形囊尾蚴的鉴定和重度感染的病理分析  

Identification and histopathology of severe infection of Cysticercus fasciolaris in Wistar rats

作　　者：张晓辉[1,2] 黄怀球[1] 钟毅[1] 冯佩英[1] 张静[3] 薛汝增[2] 李美荣[1] 陈荣章[1] 赵静[1] 

机构地区：[1]中山大学附属第三医院皮肤性病科,广东广州510630 [2]广东省皮肤性病防治中心,广东广州510500 [3]中山大学附属第二医院皮肤性病科,广东广州510120

出　　处：《热带医学杂志》2011年第5期509-511,F0003,共4页Journal of Tropical Medicine

基　　金：广东省自然科学基金(8151008901000187)

摘　　要：目的鉴定动物实验中Wistar大鼠所感染寄生虫的种属和分析其感染特征。方法解剖检查正常条件下饲养的大鼠肝脏表面的囊泡,取囊中的虫体头节以10%甲醛及KOH溶液处理后光学显微镜下进行寄生虫的形态学鉴定;并对所感染部位的肝组织进行病理学检查。结果大鼠生长状况良好,无明显异常体征,寄生的虫体鉴定为带形囊尾蚴;本次所解剖检查的Wistar大鼠对该虫的感染率高达85.7%,感染部位均为肝脏,感染虫体数目为5～120个不等,可见带形囊尾蚴早期和成熟期两种形态;肝脏病理虫体周围有嗜酸性粒细胞、淋巴细胞和浆细胞浸润,未被虫体占位的肝小叶结构正常,未见肝脏纤维瘤样变。结论 Wistar大鼠在饲养中易反复感染带形囊尾蚴,并能耐受重度感染,作为动物模型,一方面应严防该虫的感染对实验结果的干扰,另一方面可作为一个很好的免疫应答的干预模型应用于其他疾病的研究。Objective To investigate the Cysticercus fasciolaris infection in the Wistar rats in animal experiment and identify the genera of the parasite.Method All rats normally fed were dissected.Scolex of the parasite was observed under the light microscope after treated with 10% formaldehyde and KOH solution.Results The growth condition of the rats was good,but 85.7% of rats were infected with Cysticercus fasciolaris.The parasite was only observed in liver,and the number ranged from 5 to 120.Histopathology of liver sections revealed a polypide surrounded by eosinophilic granulocytes,lymphocytes and plasma cells.Lobular architecture was intact.Conclusion Wistar rat was easy to get infected with Cysticercus fasciolaris repeatedly and have a tolerance characteristic for severe infection.As an animal model,researchers must keep Wistar rat free of Cysticercus fasciolaris to obtain accurate experimental data.On the other hand,the infected Wistar rat might be a good intervention model for immune response to apply to other diseases.

关 键 词：大鼠 带形囊尾蚴 鉴定 病理 

分 类 号：R383.34[医药卫生—医学寄生虫学]