乙胺丁醇体外诱导结核分枝杆菌耐药性的实验研究  

Drug resistance of Mycobacterium tuberculosis induced by ethambutol in vitro

作　　者：梁建琴[1] 李洪敏[1] 王金河[1] 陈志[1] 冯士生[1] 杨海英[1] 

机构地区：[1]解放军第309医院全军结核病研究所,北京100091

出　　处：《中华老年多器官疾病杂志》2011年第2期104-106,共3页Chinese Journal of Multiple Organ Diseases in the Elderly

摘　　要：目的研究乙胺丁醇(EMB)体外诱导结核分枝杆菌耐药性,指导临床合理用药。方法对结核分枝杆菌H_(37)Rv实施乙胺丁醇耐药性诱导试验,对诱导菌侏和34株耐药临床分离株进行聚合酶链反应-单链构象多态性(PCR-SSCP)和测序分析(DS)。结果 12株耐药临床分离株和第8代H_(37)Rv诱导株的PCR-SSCP出现异常电泳图谱,DS证实发生embB306 ATG→GTG突变。结论长期间歇低浓度药物刺激能够诱导结核分枝杆菌获得性耐EMB。Objective To study the drug resistance of Mycobacterium tuberculosis induced by ethambutol in vitro, and to guide the selection and use of anti-tuberculous agents in clinical practice. Methods The induced experiments of drug resistance on Mycobacterium tuberculosis H37Rv by ethambutol in vitro were performed. The induced strains and 34 ethambutol-resistant strains were analyzed by polymerase chain reaction-single stranded conformation polymorphism （PCR-SSCP） and PCR-direct sequencing （PCR-DS） techniques. Results The SSCP spectrum in 12 ethambutol-resistant strains and the induced strains of eighth generation was different from that of wild type H37Rv. PCR-DS showed the mutation from ATG to GTG at codon 306 of embB. Conclusion The acquired ethambutol-resistance could be induced by long term exposure to the low level of intermittent antibacterial agents.

关 键 词：分枝杆菌 结核 乙胺丁醇 抗药性 细菌 

分 类 号：R378.91[医药卫生—病原生物学]