结核抗体检测在老年肺结核诊断中的应用价值  

Antibody detection for diagnosis of tuberculosis in elderly patients

作　　者：马秀清[1] 陈良安[1] 李春笋[1] 于玲[1] 梁媛[1] 

机构地区：[1]解放军总医院呼吸科,北京100853

出　　处：《中华老年多器官疾病杂志》2011年第2期146-148,共3页Chinese Journal of Multiple Organ Diseases in the Elderly

摘　　要：目的探讨结核抗体检测在老年肺结核诊断中的应用价值。方法回顾性分析296例老年住院患者血清结核抗体的结果。以老年肺结核的临床诊断为金标准,将一步免疫层析法、斑点免疫金渗滤法、免疫印迹法以及传统检测方法的敏感度和特异度同金标准进行比较,同时比较了41例老年肺结核病患者与83例中青年肺结核病患者血清结核抗体的检测结果。结果一步免疫层析法和免疫印迹法检测老年血清结核抗体的敏感度和特异度分别为58.54%和93.43%,75.61%和89.39%,与金标准检测结果相比,差异无统计学意义(P>0.05)。三种方法在老年和中青年肺结核病患者中血清结核抗体检测的敏感度无统计学差异(P>0.05)。结论一步免疫层析法和免疫印迹法与金标准检测结果一致,且敏感度高,特异度好,操作简单、快捷,不受年龄的影响,适合老年住院患者结核病的辅助诊断。Objective To investigate the value of antibody detection for the diagnosis of tuberculosis in elderly patients. Methods We retrospectively analyzed the results of serum mycobacterium tuberculosis antibody （TB-Ab） tests of 296 elderly in-patients. Clinical diagnosis of tuberculosis was taken as gold standard. The sensitivity and specificity of immunochromatography, dot-immunogold filtration assay （DTBA）, immunoblotting, and traditional detection method were compared with that of gold standard. We also compared the serum TB-Ab detection results between 83 young or middle-aged patients and 41 elderly patients. Results The sensitivity and specificity of immunochromatography and immunobloting were 58.54% and 93.43%, 75.61% and 89.39% respectively. There was no sigificant difference with gold standard （P 〉 0.05）. There was also no significant difference in sensitivity of the different detection methods between elderly patients and young or middle-aged patients （P 〉 0.05）. Conclusion Immunochromatography and immunoblotting are in consistent with gold standard in detection of TB-Ab. They are all sensitive, specific, simple, and fast to manipulate The results are independent of patient＇s age. They are suitable for diagnosis of tuberculosis in elderly in-patients.

关 键 词：分枝杆菌 结核 免疫层析法 斑点免疫金渗滤法 免疫印迹法 

分 类 号：R520.4[医药卫生—内科学]