改性壳聚糖包载的磁性紫杉醇纳米微粒的制备及对恶性脑胶质细胞瘤的抑制作用  

Preparation of Superparamagnetic Paclitaxel Nanoparticles from Modified Chitosan and Their Cytotoxicity against Malignant Brain Glioma

作　　者：赵明[1] 李安民[1] 常津[2] 王汉杰[2] 梁树立[1] 张嘉靖[1] 闫润民[1] 

机构地区：[1]解放军总医院第一附属医院神经外科,北京100037 [2]天津大学材料科学与工程学院纳米生物技术研究所,天津300072

出　　处：《生物医学工程学杂志》2011年第3期513-516,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30772245);解放军总医院科技创新苗圃基金资助项目(09KMM27)

摘　　要：采用改性壳聚糖包载Fe3O4磁流体和紫杉醇形成纳米微粒悬浮液,用透射电镜(TEM)观察微粒形态和粒径,利用震动样本磁强仪(VSM)检测其顺磁性,用流式细胞法检测其对人脑星形胶质母细胞瘤细胞系U251细胞凋亡诱导作用。结果显示所制得的超顺磁性紫杉醇纳米微粒混悬液呈黑色,无絮状物或沉淀,稀释后呈棕色透明。电镜下微粒呈球形,分散性好,平均有效粒径为15 nm,并具有超顺磁性。流式细胞术检测表明纳米微粒对U251细胞有显著细胞毒性作用,其凋亡率与紫杉醇相当(P>0.05)。这表明纳米紫杉醇磁性微粒不仅能有效增加紫杉醇的水溶性,同时具备超顺磁性,并能有效杀伤脑胶质瘤细胞,为进一步脑胶质瘤的磁靶向化疗奠定基础。We synthesized the superparamagnetic paclitaxel nanoparticles from modified chitosan tangling around Fe_3O_4 ferrofluid and taxol,and observed the nanoparticles with transmission electronic microscopy（TEM）.Then we evaluated the paramagnetism of the particles by vibration specimen magnetometer（VSM） and tested their cytotoxicity with flow cytometry（FCM）.The prepared nanoparticle solution was black without any floccule or sediment and appeared transparent after diluted.The nanoparticles were spherical and dispersed in water with mean diameter of 15nm under TEM and showed superparamagnetic character.FCM test showed the nanoparticles had significant toxic effects against malignant astrocytoma U251 cell lines,equal to taxol alone.These results showed that the superparamagnetic nanoparticle not only enhanced the solubility of paclitaxel in water,but also was superparamagnetic and cytotoxic,which make suitable tools for magnetic targeting chemotherapy of brain gliomas.

关 键 词：壳聚糖 纳米 磁导向 紫杉醇 胶质瘤 

分 类 号：R943[医药卫生—药剂学] R96[医药卫生—药学]