硝酸盐还原试验快速检测结核分枝杆菌药敏试验  

Rapid detection of drug susceptibility testing in Mycobacterium tuberculosis by Nitrate reductase assay

作　　者：罗丹[1] 向延根[1] 潘建华[1] 王雷[1] 

机构地区：[1]长沙市中心医院检验科,湖南长沙410004

出　　处：《临床肺科杂志》2011年第7期1046-1047,共2页Journal of Clinical Pulmonary Medicine

基　　金：国家十一五科技重大专项(艾滋病和病毒性肝炎等重大传染病防治)(No2009ZX10005-018);湖南省卫生厅科技基金(NoB2008074)

摘　　要：目的探讨一种廉价、简便、快速的检测结核分枝杆菌药敏的方法。方法以绝对浓度法对照,对92株结核分枝杆菌进行硝酸盐还原酶试验,并比较两种方法。结果四种一线抗痨药物利福平、异烟肼、链霉素、乙胺丁醇的敏感度分别为96.83%、97.22%、94.11%、88.89%;特异度为92.59%、85.0%、87.80%、96.43%;符合率为93.48%、96.74%、91.30%、93.48%。两种方法无显著性差异,但硝酸盐还原酶法的检测时间要比绝对浓度法快2～3周。结论硝酸盐还原酶法是一种快速、准确检测结核药敏的方法。Objective To investigate a kind of low-priced,convenient way for druo susceptibility testing of Mycobacterium tuberculosis.Methods 92 Mycobacterium tuberculosis strains was determined by using abosulute concentration method and NRA.Results The sensitivity and specificity of the four drugs were 96.83% and 92.59% for rifampin,97.22%and 85.0% for isoniazid,94.11% and 87.80% for streptomycin,88.89% and 93.48% for hydrochlorid.The accordance rates of these two methods were 93.48%,96.74%,91.30%,93.48%,respctively.There was no obvious difference among two metheds,but NRA can be ahead of the absolute concentration method about 2-3 weeks.Conclusions NRA is rapid and accurate drug susceptibility testing of Mycobacterium tuberculosis.

关 键 词：结核分枝杆菌 硝酸盐还原酶试验 药物敏感试验 

分 类 号：R446.5[医药卫生—诊断学]