解剖M型超声心动图对骨髓间充质干细胞移植前后心脏结构和功能的研究  

A Study on Left Ventricular in Structure and Function before and after Mesenchymal Stem Cells Transplantation to Myocardial Infarction by Anatomic M-mode Echocardiography

作　　者：张军辉[1] 李媛[1] 曹礼庭[1] 张小明[1] 董国礼[1] 顾鹏[1] 刘兴华[1] 张青[1] 

机构地区：[1]医学影像学四川省重点实验室川北医学院附属医院超声科,四川南充637000

出　　处：《川北医学院学报》2011年第3期243-245,共3页Journal of North Sichuan Medical College

基　　金：四川省科技厅项目(05JY029-014-1)

摘　　要：目的:应用解剖M型超声心动图(AME)对猪急性心肌梗死(AMI)经骨髓间充质干细胞(MSCs)移植治疗前后左心室结构、局部及整体功能进行评价。方法:采用经冠状动脉丝线堵塞法制作小型猪AMI动物模型,造模2周后,经皮冠状动脉行移植MSCs治疗,于造模前、移植前后检测左心室16节段室壁运动搏幅(Am),左心室舒张末期内径(LVEDd)。单平面Simpison法测量左心室射血分数(LVEF)。根据造模前Am的95%可信区间下限值(6 mm)判定室壁运动异常节段(心肌缺血/梗死节段)。结果:造模前-移植前-移植后室壁运动异常节段数表现为无-有-减少的变化;LVEDd有正常-增大-减小趋势;LVEF为正常-减低-增加趋势。结论:AME可以较好地评价AMI的部位及MSCs移植前后左心室的结构与功能。Objective： To assess the structure,the global and local function of left ventricular before and after mesenchymal stem cells（MSCs） transplantation to acute myocardium infarction（AMI） in pigs by anatomic M-mode echocardiography（AME）.Methods： The coronary arteries of pigs were obstructed by thread to prepare model AMI.After two weeks,MSCs were transplanted to the coronary arteries.AME was performed before the procedure,before and after the transplantation.The parameters were detected including the wall motion amplitudes（Am） of left ventricular 16 segments,left ventricular end diastolic diameter（LVEDd）,left ventricular ejection fraction（LVEF） was measured with Simpson single-plane methods.The lower limit value（6mm） of 95% confidence interval of the difference about Am in healthy pigs was regarded as the standard for diagnosing abnormal segments（ncreasin segments）.Results： Before the procedure,before the transplantation,after the transplantation,the number of abnormal segments displayed without-having-reducing;LVEDd presented normal-increaseing-decreasing;LVEF presented normal-decreasing-increasing.Conclusion： AME is able to more accurately detect the place of AMI and assess the structure,the global and local function of left ventricular before and after MSCs transplantation.

关 键 词：解剖M型超声心动图 骨髓间充质干细胞 心肌梗死 心室结构 功能 

分 类 号：R445.1[医药卫生—影像医学与核医学]