不同运动负荷对大鼠海马组织NGF和nNOS表达的影响及其意义  

Influence of different exercise load on expression of NGF and nNOS in rat hippocamp tissues and significances

作　　者：刘忠民[1] 刘敬阳[1] 赵立君[1] 刘英伟[1] 孙茹 姜云鹏[2] 

机构地区：[1]吉林大学体育学院运动人体科学研究室,吉林长春130012 [2]吉林大学白求恩医学院病理解剖学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2011年第3期437-440,I0002,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(201015115)

摘　　要：目的:探讨不同运动负荷对大鼠海马组织神经生长因子(NGF)和神经型一氧化氮合酶(nNOS)表达的影响及机制,为运动干预脑健康及预防脑老化研究奠定基础。方法:40只Wistar雄性大鼠随机分为不进行运动干预的对照组和每天进行15、30和60 min的小、中、大游泳运动负荷的实验组,实验8周后取大鼠脑组织,免疫组织化学S-P法检测大鼠海马组织NGF和nNOS表达水平及形态学表现。结果:与对照组比较,小、中、大运动负荷组大鼠海马组织NGF阳性表达水平均明显升高(P<0.05,P<0.01);与小运动负荷组比较,中、大运动负荷组大鼠海马组织NGF阳性表达水平升高(P<0.01),但中等运动负荷组大鼠与大运动负荷组比较差异无统计学意义(P>0.05)。与对照组比较,中、大运动负荷组大鼠海马组织nNOS阳性表达水平升高(P<0.01);与小运动负荷组比较,中、大运动负荷组大鼠海马组织nNOS阳性表达水平升高(P<0.05,P<0.01),大运动负荷组大鼠海马组织nNOS阳性表达水平高于中运动负荷组(P<0.01)。免疫组织化学染色:大鼠海马组织NGF阳性神经元主要表达在神经细胞胞浆,呈棕色;nNDS阳性神经元呈棕褐色,着色主要位于胞浆。结论:大脑海马组织NGF和nNOS的表达水平在一定范围内随着运动负荷的增加而升高,提示在不同的运动负荷阶段NGF和nNOS对海马神经的生长和损伤的修复具有重要作用。Objective To explore the influence of different intensities of exercise load on the expressions of nerve growth factor（NGF）and neuronal nitric oxide synthase（n-NOS） in rat hippocamp tissues and mechanism and lay a foundation for study on prevention of brain diseases.Methods 40 Wistar male rats were divided into non-exercise group（control group） and exercise group.In exercise group,the rats swam for 15,30 and 60 min every day.After 8 weeks,the rat brain was obtained,the expression levels of NGF and n-NOS in rat hippocamp tissues were detected with S-P immunohistochemistry.Results Compared with control group,the positive expression levels of of NGF in rat hippocamp tissues in 15,30,and 60 min groups were significantly increased（P0.05,P0.01）.Compared with 15 min group,the positive expression levels in 30 and 60 min groups were increased（P0.01）;but there was no significant different between 30 min group and 60 min group（P0.05）.Compared with control group,the nNOS positive expression levels in rat hippocamp tissues in 30 and 60 min groups were significant increased（P0.01）.Compared with 15 min group,the nNOS positive expression levels in rat hippocamp tissues in 30 and 60 min groups were significantly in creased（P0.05,P0.01）,and there was significant difference between 30 min group and 60 min group（P0.01）.Conclusion The expression levels of NGF and nNOS in rat hippocamp tissues are increased with the increase of exercise load within a certain range.It indicates that the NGF and nNOS in different periods with excise load have important effects on in the growth and repair of hippocampal nerve.

关 键 词：运动负荷 海马 神经生长因子 一氧化氮合酶 

分 类 号：R332[医药卫生—人体生理学] R87[医药卫生—基础医学]