吉林省双阳梅花鹿鹿茸水溶性蛋白组分的提取及对小鼠主要脏器病理形态学的影响  

Extraction of protein components from Jilin Shuangyang sika deer pilose antler and their effects on pathomorphology of major organs in mice

作　　者：赵大玮[1] 李丹[2] 陈智嘉[2] 魏征人[2] 吴荻[1] 

机构地区：[1]吉林省肿瘤医院乳腺科,吉林长春130012 [2]吉林大学白求恩医学院药理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2011年第3期448-451,I0003,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目资助课题(20070927-03,200705446)

摘　　要：目的:探讨吉林双阳梅花鹿鹿茸水溶性蛋白组分应用的安全性,为鹿茸蛋白的开发应用提供实验依据。方法:将新鲜双阳梅花鹿鹿茸进行高速组织匀浆,离心取上清,用饱和硫酸铵进行沉淀,PBS溶解沉淀,最后通过SephadexG25分子筛层析方法过滤除盐,初步获取鹿茸水溶性蛋白组分。将58只ICR小鼠随机分为生理盐水灌胃组、鹿茸蛋白溶液灌胃组、鹿茸蛋白溶液皮下注射组和鹿茸蛋白溶液腹腔注射组,按6.7 mg.kg-1给药,每天2次,第28天处死小鼠,取心、肝、肾和肺组织进行病理形态学观察。结果:鹿茸总蛋白溶液采用Sephadex G25层析过滤除盐后,平均浓度为0.685 g.L-1。病理形态学切片显示:鹿茸蛋白溶液灌胃组(50.0%)、皮下注射组(91.7%)和腹腔注射组(87.5%)小鼠可见散在的心肌细胞肌浆凝聚;蛋白溶液灌胃组(40.0%)、皮下注射组(64.3%)和腹腔注射组(77.8%)小鼠肝组织有炎细胞浸润和不同程度灶状坏死;3只皮下注射组小鼠肾小管上皮有不同程度的玻璃样变性;3只蛋白溶液灌胃组小鼠有肺炎病变;皮下注射组及腹腔注射组各有3只小鼠肺间隔增宽。结论:不同给药途径的鹿茸蛋白溶液对小鼠心肌组织、肝脏组织有一定程度的病理性损害,对肾、肺组织无明显影响。Objective To evaluate the security of application of the protein components from Jilin Shuangyang sika deer antler pilose and provide experimental basis for study on its development and application.Methods The raw pilose antler from Shuangyang was homogenated with high speed.The supernatant was obtained by centrifuge.Ammonium sulfate was applied to precipitate protein solution and then PBS was used to dissolve.Sephadexg-25 was applied to eliminate irons to obtain purified total protein solution.58 ICR mice were randomly divided into control group（saline intragastric administration）,intragastric group,subcutaneous injection group,and intraperitoneal injection group all by administrating protein solution.The dosage was 6.7 mg·kg-1,twice a day.28 days later,the mice were executed and the heart,liver,kidney,lung of each group were obtained for pathological observation.Results After sephadex-G25 filtration,the concentration of total protein solution was 0.685 g·L-1.Myoplasm condensation was found in intragastric group（50.0%）,subcutaneous injection group（91.7%） and intraperitoneal injection group（87.5%）.Inflammatory cell infiltration and focal necrosis were observed in intragastric injection group（40.0%）,subcutaneous injection group（64.3%） and intraperitoneal injection group（77.8%）.3 cases of hyaline degeneration were found in subcutaneous injection group.Every 3 cases of pneumonia were found in intragastric injection group.Every 3 cases of broaden pulmonary septum were found in subcutaneous injection group and intraperitoneal injection group.Conclusion Antler protein of sika deer can result in toxication of heart and liver of mice in some extent,but has no significant effect on either kidney or lung.

关 键 词：鹿茸蛋白 病理改变 小鼠 

分 类 号：R965.3[医药卫生—药理学]