紫外分光光度法测定尿液中加替沙星的含量  

Determination of Gatifloxacin Level in Urine with Ultraviolet Spectrophotometry

作　　者：周萍[1] 肖芬[1] 李好枝[1] 

机构地区：[1]大理学院药学与化学学院,云南大理671000

出　　处：《大理学院学报（综合版）》2011年第6期4-6,共3页Journal of Dali University

摘　　要：目的：建立尿液中加替沙星的含量测定方法。方法：采用紫外分光光度法，以333nm作为检测波长。通过5名健康志愿者实验，考察尿药中加替沙星的排泄量。结果：尿液中加营沙星在1．00～36．00μg／mL浓度范围内线性关系良好（r=0．9998），平均方法回收率为98．1％，RSD为2．87％；平均萃取回收率为9514％，RSD为3．66％，24h排泄量在44-3％～69．6％。结论：该方法简单、快速、灵敏、准确，可推广使用。Objective： To determine the content of Gatifloxacin in urine with ultraviolet speetrophotometry method. Methods： Urine samples from 5 healthy volunteers were collected .The contents of Gatifloxacin in urine were determined by ultraviolet speetrophotnmetry with the detective wavelength of 333nm. Results： Data have shown a good linear relation between Gatifloxacin cotent and absorbance （r =0.999 8）with the Gatifloxacin concentration in 1.00 to 36.00 μg/ml. The average recoveiy was 98.1% with RSD 2.87%; the average extraction recovery was 95.4% with RSD 3.66%, excretion rate in 24 hours was 44.3%-69.6%. Conclusion： The simple, quick, sensitive and accurate method applied here is worthy of promoting in Gatifloxacin determine in urine.

关 键 词：加替沙星 紫外分光光度法 含量测定 

分 类 号：R978.1[医药卫生—药品]