核转录因子κB与肿瘤坏死因子-α对子宫内膜异位的影响  

Effects of NF-κB and TNF-α on Endometriosis

作　　者：张静华[1] 李晓红[1] 李海燕[1] 

机构地区：[1]大理学院附属医院,云南大理671000

出　　处：《大理学院学报（综合版）》2011年第6期51-53,共3页Journal of Dali University

摘　　要：目的:检测核转录因子(NF-κB)在子宫内膜异位症(EM)患者异位内膜及在位内膜组织中的表达,以及其血清中肿瘤坏死因子-α(TNF-α)的变化,探讨NF-κB与TNF-α在EM发病机制中的作用。方法:采用酶联免疫吸附试验(ELISA)测定TNF-α的表达,免疫组化SP法检测NF-κB的表达情况。结果:子宫内膜异位症(EM)组血清TNF-α含量显著高于对照组(P<0.01),且表达量随病情加重有上升趋势(P<0.05)。EM患者中异位内膜组NF-κB表达强度显著高于在位组及对照组(P<0.01)。结论:NF-κB与TNF-α在EM患者异位内膜中的异常高表达与EM的发病有密切关系。Objective： The expression of NF- κB in the tissue of endometria and tumor necrosis factor-α in serum in endometriosis patients were measured to investigate the relationship between NF- κB, TNF-α and endometriosis. Methods： Enzyme linked immunosorbent assay method was applied to detect the the expression of TNF-α. The expression of NF-κB was detected by immunohis tochemistry method. Results： The TNF-α serum level of endometriosis patients was significantly higher compared to the normal control （P 〈 0.01 ）. The expression of NF- κB was major located in epithelioglandular kytoplasm of trusion-endometriosis, and the expression of NF-κB during progestational stage was higher than that in proliferative stage （ P 〈 0.05 ）. Conclusion： The abnormal expression of NF- κB and TNF-α were related to endometriosis.

关 键 词：子宫内膜 子宫内膜异位 NF—κB 肿瘤坏死因子-Α 

分 类 号：R711.74[医药卫生—妇产科学]