Ghrelin受体表达对大鼠胃肠手术后小肠动力不足的影响  

Effect of the expression of ghrelin receptors on the postoperative underpowered small intestinal motility in rats

作　　者：杨成广[1] 于嵩[1] 王志刚[1] 郑起[1] 

机构地区：[1]上海交通大学附属第六人民医院普通外科,200233

出　　处：《中华胃肠外科杂志》2011年第6期455-458,共4页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的探讨ghrelin受体变化对大鼠术后小肠动力不足的影响。方法采用体外肌条实验观察在卡巴胆碱（10nmol／L）存在和缺乏时，不同浓度的ghrelin（0、0．01、0．1、0．5、1．0μmol／L）对肌条收缩力的影响。采用免疫组织化学方法和Westemblot技术检测行端侧肠吻合的实验组大鼠和行假手术的对照组大鼠小肠肌层ghrelin受体（GHS—R1a）的表达情况。结果在体外，卡巴胆碱存在时，ghrelin能够增强小肠平滑肌肌条的收缩，不同浓度的ghrelin（0．1、0．5、1．0la,mol／L）引起的收缩力差异具有统计学意义[（223±18）％、（245±22）％、（264±25）％，P〈0．01]。免疫组化染色显示．GHS—R1a主要分布于小肠肌层．实验组大鼠小肠环形肌和纵行肌中GHS—R1a表达均弱于对照组。Westernblot结果显示，实验组小肠ghrelin受体表达量（0．51±0．02）明显低于对照组（0．71±0．01，P〈0．01）。结论术后小肠肌层ghrelin受体表达下调所导致的ghrelin效应减弱可能参与术后小肠动力不足的发生。Objective To investigate the effect of the expression of ghrelin receptors on the postoperative small intestine dysmotility in rat models. Methods The effect of different concentrations of ghrelin （0, 0.01, 0.1, 0.5, 1.0 μmol/L） on the contraction of smooth muscle strips of rat small intestine in the presence or absence of carbachol was observed in vitro. End-to-side anastomosis was performed in the study group and sham controls were used. The expression of ghrelin receptors （GHS-Rla） in small intestine muscle layers was detected by immunohistochemistry and Western blot. Results In vitro, ghrelin enhanced the contraction of smooth muscle strips in the presence of carbachol, and the differences in contraction induced by different concentrations of ghrelin（0.1, 0.5, 1.0μmol/L） were statistically significant [（223±18）%, （245±22）%, （264±25）%, P〈0.01]. Immunohistochemistry study showed that GHS-Rla mainly located in the muscular layer of the bowel wall. The expression of GHS-Rla in the circular and longitudinal muscle was significantly weaker than that in the control group. The expression of ghrelin receptors after surgery was down-regulated in the study group, which was lower than that in the control group（0.51±0.02 vs. 0.71±0.01, P〈0.01）. Conclusion Down regulation of ghrelin receptors in small intestine muscle layers may contribute to the occurrence of small intestine dysmotility after intestinal surgery.

关 键 词：受体 ghrelin 小肠动力 模型 大鼠 

分 类 号：R656[医药卫生—外科学]