多抗原肽免疫血清体外阻断丙型肝炎病毒感染外周血单个核细胞的研究  

Blocking hepatitis C virus infection of peripheral blood mononuclear cells in vitro by rabbit sera against hypervariable region 1 of hepatitis C virus

作　　者：闵峰[1] 王素美[1] 黄文琪[1] 

机构地区：[1]福建省厦门市解放军第174医院感染病科,361003

出　　处：《实用肝脏病杂志》2011年第3期164-166,共3页Journal of Practical Hepatology

摘　　要：目的探讨多抗原肽(MAP)免疫兔血清体外阻断丙型肝炎病毒(HCV)感染人外周血单个核细胞。方法用兔抗MAP血清按一定比例混合后接种于HCV感染的人外周血单个核细胞,采用逆转录聚合酶链反应、免疫组化和原位杂交技术分别检测细胞内或上清中HCV RNA、细胞内HCV NS3和HCV NS5抗原的表达。结果兔抗MAP血清按1:1混合后,外周血单个核细胞仍可检出HCV RNA,细胞有HCV NS3抗原表达;而按5:1和10:1混合后,单个核细胞内未检测出HCV正负链RNA,未见特异性抗原表达,原位杂交也未检测出HCV RNA存在。结论兔抗MAP血清在体外能部分阻断HCV感染。Objective To investigate whether an anti-multiple antigen peptide（MAP）rabbit serum against hypervariable region 1 of hepatitis C virus（HCV）can block viral infection of peripheral blood mononuclear cells in vitro.Methods An anti-MAP rabbit serum was mixed with positive sera of HCV RNA respectively in some series of proportions.The cells and supernatant were harvested at various phases during the cultures.Both the plus and minus strands of HCV RNA in infected cells or supernatants were examined by reverse transcription polymerase chain reaction（RT-PCR）.The HCV NS3 and HCV NS5 antigens in infected cells were respectively detected with the monoclonal antibodies to them by immunohistochemical assay.The minus strand of HCV RNA in infected cells were localized by in situ hybridization.Results When the anti-MAP rabbit serum and infectious serum（1：1）were added into peripheral blood mononuclear cells,HCV RNA could be detected in culture cells,and the positive signals of NS3 and NS5 antigens could be detected within cytoplasm of infected cells.The minus strand of HCV RNA was located within cytoplasm.But when the anti-MAP rabbit serum and infectious serum（5：1 and 10：1）were added into peripheral blood mononuclear cells,the HCV RNA,NS3 and NS5 antigens were negative in the infected cells.The minus strand of HCV RNA was not found in the cytoplasm of the cells.Conclusions The anti-MAP rabbit serum could partly prevent the cultured cells from being infected by HCV.

关 键 词：丙型肝炎病毒 细胞培养 多抗原肽 免疫血清 

分 类 号：R392[医药卫生—免疫学]