辅助生殖技术治疗后孕早期自然流产的绒毛细胞染色体分析  

Chromosomal Analysis in Spontaneous Abortion during the First Trimester after Assisted Reproduction Technique Treatment

作　　者：李跃萍[1] 徐雯[1] 黎明红[1] 麦扬青[1] 陈竞茜[1] 闫庆峰[2] 

机构地区：[1]海南医学院附属医院生殖医学科,海南海口570102 [2]海南医学院附属医院外科,海南海口570102

出　　处：《昆明医学院学报》2011年第5期50-53,共4页Journal of Kunming Medical College

摘　　要：目的了解辅助生殖技术(ART)治疗后孕早期自然流产的绒毛细胞染色体的异常情况,为优生优育工作作指导.方法选择行体外受精-胚胎移植(IVF-ET)、单精子卵胞浆内显微注射(ICS)I、冻融胚胎移植(F-ET)及宫腔内人工授精(IU)I治疗后的自然流产绒毛标本41例,进行绒毛细胞培养及G显带,分析染色体核型.结果 41例标本中有30例培养成功,绒毛染色体异常15例,占50%.多数为数目异常,其中X单体1例,染色体三体11例(包括18三体1例,16三体3例,15三体2例,4号三体1例,9号三体1例,2号三体1例,嵌合体型21三体2例),多倍体1例,具有额外小染色体的核型1例.结论 ART妊娠流产与胚胎染色体异常关系密切,非整倍体是导致ART治疗后自然流产的重要原因.Objective To know about chromosome abnormalities in spontaneous abortion during the first trimester after assisted reproduction technique treatment and to provide some data for eugenics.Methods A retrospective analysis was performed on 41 clinical spontaneous abortion cases during the first trimester following ART,which including in vitro fertilization-embryo transfer（IVF-ET）and intracytoplasmic sperm injection（ICSI）and frozen embryo transfer（F-ET）and intrauterine insemination（IUI）.Cytogenetic analysis of the chorionic villi by cell culture and standard G-banding cytogenetic techniques was performed.Results Successful analysis was conducted in 30 specimens.Fifteen of 30 specimens had a chromosomal abnormality,accounting for 50%.The majorities were numerical abnormalities such as monosomy X（one case）.Trisomies for chromosomes 11 cases,including 18 trisomies in one case,16 trisomies in three cases,15 trisomies in two case,4 trisomies in one cases,9 trisomies in one case,2 trisomies in one case,mosaic trisomy 21 trisomies in two cases,and polyploidin one case were observed.46,XX/47,XX,＋mar karyotype was observed in one case.Conclusions ART abortion is closely related with embryo chromosome abnormalities.Aneuploidy is the major factor affecting embryonic development in spontaneous abortions during the first trimester after ART.

关 键 词：辅助生殖技术 孕早期 自然流产 

分 类 号：R715.5[医药卫生—妇产科学]