钛板和死骨表面细菌生物膜模型的建立及观察  

Establishment and observation of bacterial biofilm on surfaces of titanium plate and sequestrum

作　　者：冯安平[1] 孙效棠[2] 王万明[2] 牛永永[1] 黄少斌[1] 

机构地区：[1]福建中医药大学 [2]南京军区福州总医院骨二科,福州市西二环北路156号350025

出　　处：《中国矫形外科杂志》2011年第12期1030-1033,共4页Orthopedic Journal of China

基　　金：福建省自然科学基金资助项目(编号:2009J05082)

摘　　要：[目的]探讨所建立的钛板和死骨表面的细菌生物膜(BBF)模型用于研究同种细菌在不同异物表面的BBF形成能力的可行性,从而指导慢性骨髓炎的治疗。[方法]采用改良的置片培养法建立钛板和死骨表面的BBF模型。用激光共聚焦显微镜采集BBF图像及测量BBF厚度、活菌百分率。[结果]BBF是具有高度组织化的多细胞群体结构。实验组钛板和死骨的BBF厚度分别为(39.20±4.26)μm和(44.70±4.22)μm(P<0.05)。在实验组钛板表面,中层和底层活菌百分率分别为(81.50±10.46)%和(52.20±5.45)%(P<0.01)。在实验组死骨表面,中层和底层活菌百分率分别为(85.50±6.42)%和(57.30±4.92)%(P<0.01)。[结论]此次实验所建立的模型可用于研究同种细菌在不同异物表面的BBF形成能力。金黄色葡萄球菌在死骨表面的BBF形成能力比钛板强。在BBF里,底层活菌的新陈代谢比中层的慢,并且活菌大多被死菌所包裹,这种结构可能是造成慢性骨髓炎难以治愈的原因之一。[ Objective] To guide the treatment of chronic osteomyelitis via exploring whether the established model of bacterial biofilm（BBF） on surfaces of titanium plate and sequestrum can be used in the research of BBF-forming ability of the same species of bacteria on surfaces of various foreign bodies. [ Method] The modified setting piece culture method was used to establish a model of BBF on surfaces of titanium plate and sequestrum. The eonfocal laser scanning microscopy was used to collect the BBF image and measure the BBF thickness and the vital bacterium percentage. [ Result] BBF was a highly organized structure of umlticell population. In the experimental group, the mean BBF thickness was 39.20 ± 4.26 μm on surfaces of titanium plate,44.70 ± 4.22μ m on surfaces of sequestrum（P 〈 0.05）. On titanium plates of experimental group, the mean vital bacterium percentage was 81.50% ± 10.46% in the middle layer,and 52.20% ＋ 5.45% in the underlying layer（ P 〈 0.01 ）. On sequestrums of experimental group, the mean vital bacterium percentage was 85.50% ± 6.42% in the middle layer, and 57.30% ±4.92% in the underlying（ P 〈 0.01 ）. [Conclusion ] The model can be used to study BBF4orming ability of the same species of bacteria on surfaces of various foreign bodies in this experiment. BBF-forming ability of staphyloeoccus aureus on surfaces of sequestrum is higher than that of titanium plate. The metabolism of the underlying vital bacteria is slower than that of the middle layer. Most of the vital bacteria are wrapped by the dead. It may be one of the reasons that the chronic osteomyelitis is difficult to cure.

关 键 词：钛板 死骨 生物膜 激光共聚焦显微镜 

分 类 号：R687.3[医药卫生—骨科学]