头针对局灶性脑缺血大鼠海马CA3区微血管内皮基质金属蛋白酶-9影响的动态观察  

Effects of Scalp Acupuncture on Expression of Hippocampal MMP-9 in Cerebral Ischemia Injury Rats

作　　者：张慧敏[1] 韩焱晶[2] 赵万标[3] 胡凌娟[4] 费宇彤[4] 图娅[4] 

机构地区：[1]首都医科大学附属北京世纪坛医院针灸科,北京100038 [2]中国中医科学院针灸研究所,北京100700 [3]中国中医药报社,北京100085 [4]北京中医药大学,北京100029

出　　处：《针刺研究》2011年第3期193-198,共6页Acupuncture Research

基　　金：国家中医药管理局中医药留学回国人员科技活动择优资助课题(国中医药科2002LHR18);首都医科大学附属北京世纪坛医院基金项目(2007-Q4)

摘　　要：目的:动态观察头针对局灶性脑缺血模型大鼠缺血后脑微血管内皮基质金属蛋白酶(MMP-9)表达的影响,探讨头针对缺血脑组织的保护作用机制。方法:将Wistar大鼠80只随机分为正常组、假手术组各8只,缺血模型组、头针治疗组各32只,后两组又分为造模后1、3、51、0 d 4个时间段组,每时间段各8只。采用大脑中动脉线栓阻塞法建立大鼠局灶性脑缺血模型。头针组选取"百会""曲鬓"穴进行手捻针治疗。各组实验前后分别进行神经行为学评分;分别在规定的时间点处死大鼠,光镜下观察梗死局部脑组织海马CA 3区神经细胞的形态学变化;免疫组化法检测各组海马CA 3区微血管内皮MMP-9的表达。结果:模型组大鼠各时间段的神经行为学评分明显高于正常组(均P<0.01),头针10 d组的神经行为学评分明显低于同时段模型组(P<0.05)。HE染色显示,模型组海马CA 3区神经细胞出现明显的病理改变,针刺后完整的神经细胞明显增多,血管病变主要以内皮细胞肿胀、空泡形成为主。模型组MMP-9在脑缺血后1 d即开始表达,3 d逐渐增多达到高峰,5 d持续高峰,10 d开始下降,而头针各时段组缺血侧海马CA 3区MMP-9的阳性细胞表达较同时段模型组减少,其中3、5、10 d的差异有统计学意义(P<0.05,P<0.01)。结论:头针能够有效改善脑缺血状态下海马CA 3区微血管内皮MMP-9的表达;随治疗次数的增加,针刺的疗效出现累加蓄积效应。Objective To observe the effect of scalp-acupuncture on the expression of matrix metalloproteinase 9 （MMP-9） in the endothelium of the microvessels in the hippocampal CA 3 area in local cerebral ischemia （OI） rats, so as to study its underlying mechanism in improving Ol. Methods Eighty male Wistar rats were randomized into normal control （ n = 8）, sham-operation （n = 8）, CI model （n = 32）, and scalp-acupuncture （n = 32） groups. The later two groups were further equally and respectively divided into 1, 3, 5 and 10 d subgroups. CI model was established by occlusion of the middle cerebral artery （MCAO）. ＂Baihui＇（GV 20） and ＂Qubin＇（GB 7） were punctured with filiform needles and stimulated by twirling the needle at a fre- quency of about 200 revolutions/min for I min. The needles were retained for 30 min and re-manipulated once again every 10 min. The treatment was given once daily, continuously for 1, 3, 5 and 10 days respectively in different subgroups. MMP-9 immunoactivity of hippocampal CA 3 area was assayed by immunohistochemistry （SABC）. Results Following CI, the CI rats＇ neural defi- cit scores in the model and scalp-acupuncture （treatment） groups had no apparent difference（P〈0.05）. After acupuncture, the neural deficit score in the treatment group decreased gradually, and was significantly lower than that in the 10 d subgroup of model group （P〈0.05）. Compared with the 1, 3, 5 and 10 d subgroups of control group, the total area and integral optical density （IOD） values of MMP-9 immuneactivity of hippocampal CA 3 area were all increased significantly in the corresponding 4 subgroups of model group （P〈0.01）. Compared with the 3, 5 and 10 d subgroups of the model group, the total area and IOD values of MMP-9 immunoreaction positive products in the corresponding 3 subgroups of scalp-acupuncture group were decreased significantly （P〈0.05, P〈0.01）. Conclusion Scalp-acupuncture can effectively down-regulate MMP-9 expression in the hipp

关 键 词：局灶性脑缺血 头针 基质金属蛋白酶-9 神经缺损评分 

分 类 号：R245.321[医药卫生—针灸推拿学]